β-乳糖球蛋白在提功能化半孔上吸附
Laroussi Chaabane1, Camille Loupiac1, Frédéric Bouyer2
1Université Bourgogne Franche-Comté, Institut Agro, Université Bourgogne, INRAE, UMR PAM 1517, 21000 Dijon, France.
Langmuir : the ACS journal of surfaces and colloids
|July 22, 2024
概括
醇功能化的SBA-15半孔材料具有10纳米孔径,有效吸附β-乳糖球蛋白 (BLG). 这个过程是自发的,外热的,并保留了蛋白质的结构,表明了蛋白质分离应用的潜力.
科学领域:
- 材料科学 材料科学 材料科学
- 生物化学 生物化学
- 化学工程是化学工程的重要组成部分.
背景情况:
- 在吸附应用中,SBA-15半孔材料具有可调节的性能.
- β-乳糖球蛋白 (BLG) 是一种在食品和生物医学领域相关的模型球状蛋白.
- 了解蛋白质-吸附剂相互作用对于开发高效的分离技术至关重要.
研究的目的:
- 为了合成和表征具有不同孔径的大小的醇功能化SBA-15材料.
- 评估这些材料对BLG的吸附能力和动力学.
- 为了研究孔径大小和表面功能化对BLG吸附的影响.
主要方法:
- 合成具有5nm和10nm孔隙的SBA-15半孔性二氧化.
- 在SBA-15表面的醇功能化.
- 用不同的接触时间和BLG度进行吸附实验.
- 使用拉曼光谱学进行表征,并分析热力学和动力学参数.
主要成果:
- BLG的吸附能力取决于接触时间和初始BLG度.
- 醇功能化的SBA-15具有10纳米孔 (SBA-15-SH-10) 呈现出最高的吸附能力 (0.560g·g-1).
- 吸附主要发生在异质表面的毛孔内,具有自发和外热热力学.
- 在吸附过程中,醇基氧化为硫酸盐基,而不会改变BLG的二次结构.
结论:
- 孔径大小和醇功能化是SBA-15的BLG吸附能力的关键因素.
- SBA-15-SH-10是用于BLG分离的有希望的吸附剂.
- 吸附过程是高效的,并保持蛋白质的完整性,这表明实际用途.
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