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Updated: Jul 8, 2026

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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用于监测细胞NADPH动态的化胺基化学遗传传感器

Huimin Chang1, Simon Clemens2, Pingting Gao1

  • 1Key Laboratory of Smart Drug Delivery Ministry of Education, School of Pharmacy, Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Zhangheng Road 826, Shanghai 201203, China.

Journal of the American Chemical Society
|July 22, 2024
PubMed
概括

研究人员开发了一种新型FOCS-NADPH生物传感器,用于实时监测活细胞中的尼古丁胺氨基二核酸盐 (NADPH). 这种创新工具为研究细胞代谢和NADPH动态提供了一种强大的新方法.

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...