在不同的光周期下,对Tetrastigma hemsleyanum中的CONSTANS-LIKE基因的参考基因和表达模式的评估
Gang Zhao1, Wenling Liu1, Guowei Lin1
1Life and Science College, Shangrao Normal University, Shangrao 334000, P. R. China.
Functional plant biology : FPB
|July 22, 2024
概括
光周期影响了Tetrastigma hemsleyanum根的形成. 常态 (COL) 基因表达模式,特别是ThCOL4和ThCOL5,随着光周期的变化而变化,这表明它们在不同的生物过程中发挥了作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
背景情况:
- 常态相似 (COL) 基因是调节植物生长和发育以应对光周期的关键信号分子.
- 光周期显著影响Tetrastigma hemsleyanum根茎的形成.
研究的目的:
- 为了研究不同光周期条件下的T. hemsleyanum叶子中COL基因的振荡模式和表达特征.
- 在T. hemsleyanum中确定基因表达分析的稳定基因参考基因.
主要方法:
- 使用定量逆转录PCR (qRT-PCR) 来分析基因表达.
- 使用GeNorm,NormFinder和BestKeeper软件来评估候选参考基因 (18S rRNA,TUBA,30S rRNA,TBP,EF-1α,RPII) 的稳定性.
- 两种ThCOL基因 (ThCOL4和ThCOL5) 从T. hemsleyanum转录组库中进行了选.
主要成果:
- 表达稳定性分析确定EF-1α,TUBA和18SRNA作为合适的参考基因.
- 在不同的光周期下,ThCOL4和ThCOL5的表达峰值发生在不同的时间.
- 光周期显著影响了ThCOL4和ThCOL5.5的振荡模式.
结论:
- 光周期在T. hemsleyanum中差异调节ThCOL4和ThCOL5的表达.
- 这两个ThCOL基因可能参与了不同的光周期依赖的生物过程,可能与根茎的形成有关.
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