Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Exome sequencing directly implicates 68 genes in inflammatory bowel disease.

medRxiv : the preprint server for health sciences·2026
Same author

Gut microbial signatures and stability are associated with a co-diagnosis of endometriosis and inflammatory bowel disease.

iScience·2026
Same author

Neglected kingdoms: the gut virome, mycobiome and their role in inflammatory bowel disease.

Gut microbes·2026
Same author

The Gut Microbiome at the Onset of Inflammatory Bowel Disease: A Systematic Review and Unified Bioinformatic Synthesis.

Gastroenterology·2025
Same author

Gut microbial-derived 3,4-dihydroxyphenylacetic acid ameliorates reproductive phenotype of polycystic ovary syndrome.

iMeta·2025
Same author

DNA reference reagents isolate biases in microbiome profiling: a global multi-lab study.

mSystems·2025
Same journal

MT-MRI for detection of renal interstitial fibrosis in renovascular disease.

Scientific reports·2026
Same journal

Detection of underground objects from GPR data using a lightweight YOLO-based approach.

Scientific reports·2026
Same journal

Early systemic inflammatory-metabolic trajectory phenotypes are associated with survival outcomes in metastatic renal cell carcinoma treated with nivolumab.

Scientific reports·2026
Same journal

Water balance components in a dry-seeded rice-wheat system: Untangling the effects of tillage and mulching practices.

Scientific reports·2026
Same journal

Topological approaches to quantum tensor train compression via ZX-calculus and SVD.

Scientific reports·2026
Same journal

determinants of flood impacts and adaptive capacity among market vendors in Walukuba-Masese, Jinja city, Uganda.

Scientific reports·2026
查看所有相关文章

相关实验视频

Updated: Jun 20, 2025

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System
10:16

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System

Published on: March 14, 2025

376

优化人体便样本采集用于多原子微生物群分析.

Matthew R Gemmell1, Thisun Jayawardana2, Sabrina Koentgen2

  • 1Centre for Genomic Research, University of Liverpool, Liverpool, UK.

Scientific reports
|July 22, 2024
PubMed
概括
此摘要是机器生成的。

保存便样本对于肠道微生物组研究至关重要. PSP和RNAlater缓冲器最好保持微生物和代谢概况,与冷样本密切匹配,用于准确的微生物组研究.

关键词:
我们的肠道微生物群.多个原子的分析.样品保存 样品的保存便样本的完整性是什么

更多相关视频

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

27.9K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

19.6K

相关实验视频

Last Updated: Jun 20, 2025

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System
10:16

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System

Published on: March 14, 2025

376
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

27.9K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

19.6K

科学领域:

  • 微生物学 微生物学
  • 代谢学 代谢学 代谢学
  • 基因组学就是基因组学.

背景情况:

  • 准确的肠道微生物群分析需要保持便样本的完整性.
  • 自我收集和变量处理在微生物组研究中带来了挑战.
  • 微生物群落的组成和代谢功能是健康和疾病的关键指标.

研究的目的:

  • 评估不同便样本采集/保存缓冲器的有效性.
  • 评估储存温度和时间对样本完整性的影响.
  • 为微生物组研究确定保存人类便样本的最佳方法.

主要方法:

  • 测试了三个便样本采集/保存缓冲器.
  • 在室温 (20°C),4°C和-80°C下保存样品,最长可达三天.
  • 将16S rRNA测序和短链脂肪酸概况与立即冷的样本进行比较.

主要成果:

  • 缓冲区的选择显著影响了微生物群落和新陈代谢特征.
  • 与冷样本相比,PSP和RNAlater缓冲器最准确地保存了微生物多样性.
  • 储存温度和持续时间影响了样本的完整性,但缓冲区的选择至关重要.

结论:

  • 对于人类便微生物组研究,建议使用PSP和RNAlater缓冲器.
  • 这些缓冲器确保在微生物群落和代谢资料中保持最高的保真度.
  • 优先考虑这些保存方法可以提高肠道微生物组研究的可靠性.