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登革热病毒优先使用人类和蚊子的非最佳代子

Luciana A Castellano¹, Ryan J McNamara¹, Horacio M Pallarés^1,2

¹Stowers Institute for Medical Research, 1000 E 50th Street, Kansas City, MO, 64110, USA.

Molecular systems biology

|July 22, 2024

概括

像登革热病毒 (DENV) 这样的病毒通过使用非最佳的编码子来适应,这会影响信使RNA (mRNA) 的稳定性和翻译. 这种病毒策略增强了健康状况,并塑造了宿主-病原体相互作用.

科学领域:

病毒学病毒学
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

子的最佳性影响信使RNA (mRNA) 的稳定性和翻译效率.
同义代码子在功能上是不相等的,它们起着调节作用.
登革热病毒 (DENV) 是一种蚊子传播的黄状病毒,对公共卫生有重大影响.

研究的目的:

调查病毒基因组适应宿主密码子最佳性的情况.
使用蚊子传播的登革热病毒 (DENV) 作为模型系统.
确定病毒是否优先使用非最佳的编码子,以及这如何影响健康.

主要方法:

对DENV基因组密码的使用与人类和蚊子密码的最佳性相关的分析.
在DENV感染期间检查宿主基因和tRNA表达.
在细胞培养中病毒传递期间对同名突变选择的评估.

主要成果:

在蚊子细胞中存在着子的最佳性.
DENV优先使用非最佳的编码子,并避免两个主机的最佳编码子.
宿主基因和与DENV首选编码子相关的tRNA在感染期间被上调.
病毒适应包括选择同名突变向非最佳编码子,增加适应性.
数以百计的病毒,特别是单主机病毒,对非最佳的代码子使用有偏见.

结论:

宿主-病原体相互作用塑造病毒同义词编码子选择.
DENV的非最佳密码子使用是一种适应性策略,可以增强病毒的适应性.
这些发现表明,病毒在不同的宿主中适应的更广泛的进化原理.

关键词:

在Codon Optimality中,我们可以看到Codon Optimality.登革热是因为登革热.蚊子蚊子蚊子病毒病毒病毒这是一个tRNARNA.

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