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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jun 20, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Published on: January 10, 2019

FIND-seq:用于罕见的单细胞转录组的高通量核酸细胞计.

Seung Won Shin¹, Prakriti Mudvari², Shravan Thaploo³

¹Department of Bioengineering, College of Engineering, California Institute for Quantitative Biosciences (QB3), University of California Berkeley, Berkeley, CA, USA.

Nature protocols

|July 22, 2024

概括

此摘要是机器生成的。

通过核酸检测和测序 (FIND-seq) 对细胞的定向询问,使用核酸标记物分离稀有细胞. 这种方法可以对以前难以恢复的细胞进行全转录组测序,包括完整的HIV基因组.

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

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Last Updated: Jun 20, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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Published on: January 10, 2019

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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

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Published on: October 25, 2018

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
细胞生物学细胞生物学

背景情况:

传统的细胞隔离依赖于表面蛋白标记物,限制了对罕见细胞子集的研究.
基因组和转录组数据越来越多地在计算上定义细胞子集.
基于核酸的隔离补充了现有的方法,使得人们能够获得罕见和独特的细胞群.

研究的目的:

详细说明通过核酸检测和测序 (FIND-seq) 对细胞进行集中审讯的协议.
提出检测和测序具有完整病毒基因组的罕见HIV感染细胞的协议更新.
为了使以前难以分离和测序的罕见细胞子集的机制研究.

主要方法:

开发和应用FIND-seq核酸细胞计平台.
利用RNA或DNA标记物进行罕见细胞隔离.
在隔离后进行批量或单细胞转录组分析.
检测和测序具有完整病毒基因组的罕见HIV感染细胞的协议更新.

主要成果:

FIND-seq成功地根据核酸序列分离了罕见的细胞.
该平台此前已被应用于研究XBP1在星球细胞中的剪接和HIV在CD4T细胞中的持久性.
更新的FIND-seq协议允许对具有完整病毒基因组的罕见HIV+细胞进行全转录组测序.

结论:

FIND-seq提供了一种强大的方法来隔离和研究由核酸序列定义的罕见细胞子集.
详细的协议和更新方便研究以前无法访问的细胞群.
预计这项技术将推进罕见细胞的机制研究,包括与艾滋病毒持久性相关的研究.