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单分子可视化布尼亚病毒基因组段使用光在现场杂交

Erick Bermúdez-Méndez1,2,3, Paul J Wichgers Schreur4

  • 1Department of Virology & Molecular Biology, Wageningen Bioveterinary Research, Lelystad, The Netherlands.

Methods in molecular biology (Clifton, N.J.)
|July 22, 2024
PubMed
概括

这项研究介绍了一种新的RNA光 in situ杂交 (FISH) 方法,用于可视化单个布尼亚病毒RNA段. 这项技术有助于了解病毒复制,基因组包装和组装过程.

关键词:
布尼亚病毒 (Bunyavirus) 是一种光在现场混合化中的光.光显微镜的光学显微镜.基因组包装 基因组包装通过RNA成像进行RNA成像.裂谷热病病毒病毒一个单分子分子.

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FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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