准备,识别和分子对接来自种子的两种新型抗衰老
Lingling Wang1,2, Liping Qu1,3,2, Bingwei He1,3
1Yunnan Botanee Bio-technology Group Co., Ltd., Kunming, 650106, China.
Heliyon
|July 23, 2024
概括
佩里拉种子粉通过减少活性氧物种 (ROS) 和促进皮肤细胞增殖,显示出显著的抗衰老作用. 这些生物活性为功能化品和改善资源利用提供了潜力.
科学领域:
- 生物化学 生物化学
- 皮肤病学 皮肤病学
- 食品科学 食品科学 食品科学
背景情况:
- 佩里拉种子粉是一种有价值的农业副产品.
- 蛋白质水解剂含有具有潜在健康益处的生物活性.
- 研究食品衍生的抗衰老特性可以最大限度地利用资源.
研究的目的:
- 为了研究菜种子粉蛋白质水解剂的抗氧化和抗衰老活性.
- 为了确定负责这些活动的生物活性.
- 为了探索抗衰老的分子机制.
主要方法:
- 体学和in silico生物信息学用于的识别.
- 分子对接和RT-PCR用于机制探索.
- 在体外测试中使用角质细胞 (HaCaT细胞) 来评估生物活性.
主要成果:
- 分数F2 (35 kDa) 通过抑制ROS产量和β-银酸酶活性,显示出显著的抗衰老特性.
- 两种新型,NFF和PMR,促进了角质细胞的增殖,并抑制了ROS和β-银酸酶.
- NFF和PMR降低了矩阵金属蛋白酶 (MMP) 和原体降解,强烈结合Keap1蛋白.
结论:
- 佩里拉种子粉,特别是NFF和PMR,具有强大的抗衰老特性.
- 该机制涉及Keap1结合,Nrf2释放和NF-κB通路激活.
- 这些发现支持在功能化品中应用的种子粉.
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