基于cirCHECTD1的肝细胞癌的新生存和免疫微环境相关的ceRNA调节网络的识别
Shuiqing Lan1, Guoqiang Zhong2
1Department of Pain Management, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, Guangxi, 533000, China.
Heliyon
|July 23, 2024
概括
循环RNA HECTD1 (circHECTD1) 在肝细胞癌 (HCC) 中高度表达,并充当瘤基因. 一个涉及circHECTD1的新型ceRNA网络为HCC提供了新的诊断和治疗策略.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 循环RNA HECTD1 (circHECTD1) 在肝细胞癌 (HCC) 组织中表达高.
- circHECTD1可能在HCC发育过程中起到瘤基因的作用.
- circHECTD1的特定竞争性内源RNA (ceRNA) 机制需要进一步研究.
研究的目的:
- 阐明 cirHECTD1 在 HCC 中的 ceRNA 机制.
- 构建和分析ceRNA,蛋白与蛋白相互作用 (PPI) 和与circHECTD1.1相关的关键网络.
- 根据circHECTD1 ceRNA网络,识别基于HCC的潜在诊断和治疗点.
主要方法:
- 利用多个数据库进行目标预测和数据分析,包括途径活动,免疫检查点和生存分析.
- 使用R软件进行差异基因表达,基因本体学 (GO),基因和基因组的京都百科全书 (KEGG),临床相关性,ROC曲线和单细胞分析.
- 使用Cytoscape软件构建了ceRNA,PPI和关键网络.
主要成果:
- 成功构建了ceRNA,PPI和关键网络.
- 途径丰富分析突出显示了细胞亡,细胞循环和上皮-介质酶转换 (EMT) 途径.
- 确定了与HCC存活,免疫透,免疫检查点,临床阶段和诊断相关的六个关键标,在T细胞中表达高.
结论:
- 基于cirCHECTD1的预后和免疫微环境相关的ceRNA网络已成功建立.
- 这个网络为肝细胞癌的诊断和治疗提供了新的见解.
- 这些发现表明circHECTD1是HCC的潜在生物标志物和治疗点.
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