[基于UHPLC-Q/Orbitrap HRMS与分子网络技术相结合的经典处方的Qinggu San参考样本中的化学成分的识别]
Liu-Jun Xiao1, Jia-Hao Ai1, Fei-Fei Huang1
1Ganjiang Chinese Medicine Innovation Center Nanchang 330000, China Jiangxi Provincial Key Laboratory for Pharmacodynamic Material Basis of Traditional Chinese Medicine Nanchang 330000, China.
概括
这项研究开发了一种快速的超高性能液体染色学-四极-静电场轨道轨道高分辨率质谱法 (UHPLC-Q/Orbitrap HRMS) 方法,以识别Qinggu San中的105种化学成分,包括潜在的新化合物.
科学领域:
- 分析化学 分析化学
- 自然产品化学 自然产品化学
- 药理学是指药理学,即药理学是指药理学.
背景情况:
- 经典的药物处方,如Qinggu San,需要强大的分析方法来进行质量控制.
- 了解化学成分对于阐明药理活性和确保安全至关重要.
研究的目的:
- 开发和验证一种快速分析方法,用于对金山参考样本进行全面的化学分析.
- 识别和描述古山中存在的各种化学成分.
- 为Qinggu San制剂的质量控制和药理学研究提供基础.
主要方法:
- 采用了超高性能液态染色学四极电静场轨道飞行器高分辨率质谱学 (UHPLC-Q/Orbitrap HRMS).
- 全球天然产品社会分子网络 (GNPS) 平台被用于分子网络分析.
- 鉴定是基于碎片化模式,保留时间,精确质量,碎片离子,文献和数据库.
主要成果:
- 在Qinggu San参考样本中,共识和推测了105种化学成分.
- 鉴定的成分包括化糖类,黄类,类,类和三类桑因.
- 检测到两种潜在的新化合物,突出显示了该方法的敏感性.
结论:
- 开发的UHPLC-Q/Orbitrap HRMS方法可以快速准确地分析Qinggu San. 的化学成分.
- 这种方法为Qinggu San复合制剂的质量控制提供了科学基础.
- 该方法作为分析其他传统中医药化合物制剂的宝贵参考.
关键词:
清古圣是什么意思 清古圣是什么意思化学成分 化学成分 化学成分分子网络是分子网络.参考样本是指参考样本的样本.超高性能液体染色学四极电静电场轨道飞行器高分辨率质谱学 (UHPLC-Q/轨道飞行器HRMS)更多相关视频
09:24Standardized Identification of Compound Structure in Tibetan Medicine Using Ion Trap Mass Spectrometry and Multiple-Stage Fragmentation Analysis
Published on: March 17, 2023
872
07:16Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry
Published on: February 2, 2024
546
相关概念视频
01:10Qualitative Analysis
237
Qualitative analysis is the process of identifying elements, ions, or compounds in an unknown sample. It is the first and most fundamental type of analysis based on the hierarchy of analytical goals. This hierarchy is significant as it provides a structured approach to scientific research, with qualitative analysis serving as the initial step, providing essential information before moving on to quantitative or other forms of analysis.
There are two main approaches to qualitative analysis:...
There are two main approaches to qualitative analysis:...
237
01:15High-Resolution Mass Spectrometry (HRMS)
1.3K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.3K