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Updated: Jun 20, 2025

The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies

The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies

Published on: February 12, 2015

压固体目标89Zr的生产及其用于抗体标签的应用

Anjli Shrivastav¹, Sonu Maurya¹, Manish Dixit¹

¹Department of Nuclear Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Rai-Berali Road, Lucknow, 226014, India.

Current radiopharmaceuticals

|July 23, 2024

概括

这项研究展示了一种有效的方法,用于生产-89 (89Zr) 使用压目标. 自动化净化迅速产生高纯度的89Zr,适用于正子发射断层扫描 (PET) 应用.

科学领域:

放射化学放射化学是指辐射化学.
核医学就是核医学.
材料科学材料科学材料科学

背景情况:

-89 (89Zr) 是用于正子发射断层扫描 (PET) 成像的关键正子发射放射核化物.
它的生产对于免疫PET研究至关重要,其需求是由成本有效的制造驱动的.
固体天然伊特目标通常使用,采用诸如电沉积,压和火花等离子体烧结等方法.

研究的目的:

为了研究89Zr生产的压实物准方法.
评估89Zr的手动和自动净化技术.
为了评估89Zr在化后的特定活性和纯度.

主要方法:

氧化物粉被压成颗粒,并用12 MeV的质子束进行辐射.
目标被白金,或所支持.
净化利用固体相提取与酸盐基树脂,比较手动和自动化的方法.
分析涉及马光谱和德费洛克萨 (DFO) 化,以确定特定活性.

主要成果:

使用压目标方法实现了20-30 MBq/μA.h的生产产量.
自动化净化在35分钟内产生了>98%的纯度,回收率为87.46%.
手动净化在2小时内达到>98%的纯度,回收率为91%.
使用89Zr-oxalate进行化,其纯度>98%,特异活性为25-30μCi/mmol.

结论:

压目标方法在生产高纯度的89Zr方面是有效的.
手动和自动净化方法都能提供出色的放射性核酸纯度.
用DFO进行合的成功证明了PET应用的良好标签效率和稳定性.

关键词:

按压目标的目标.化化化化含有氧酸树脂的树脂.医疗循环仪循环仪循环仪无线电标记. 无线电标记. 无线电标记. 无线电标记. 无线电标记.-89 - - -89 - - -89 - - -89 - - -89 - - -89 - - -89 - - - -89 - - - -89 - - - -89 - - - -89 -89 -89 -89 -89 -89 -89 -89 -89

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