在Brassica napus种子发育过程中对转录因子基因WRI1和LAFL的转录调节
Xu Han1, Yan Peng1, Sijie Yin1
1National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan 430070, China.
Plant physiology
|July 23, 2024
概括
这项研究确定了调节种子发育和油脂生物合成的关键基因,揭示了对转录控制的新见解,以提高作物产量.
科学领域:
- 植物分子生物学 植物分子生物学
- 遗传学 是一个遗传学.
- 农业科学 农业科学
背景情况:
- WRINKLED1 (WRI1) 和LAFL转录因子对于种子发育和油脂生物合成至关重要.
- 了解它们的转录调节是提高种子油积累的关键.
研究的目的:
- 为了全面了解 WRI1 和 LAFL 在培育大麻种子中的转录调节.
- 确定参与种子油生物合成的候选基因和调控网络.
主要方法:
- 全基因组关联研究 (GWAS) 针对302个菜加入中的WRI1和LAFL表达特征.
- 利用RNA测序,ATAC测序,极端梯度增强和Basenji模型进行基因预测.
- 通过表达分析,哈普洛型分析,双露西法酶记者和酵母单杂交试验验验证候选基因.
主要成果:
- 确定了237个表达定量特征核酸 (eQTN) 和51个eQTN与环境相互作用 (eQEI).
- 分别确定了41个和8个与eQTN和eQEI相关的候选基因.
- 预测了15个调节WRI1和LAFL表达的候选基因,其中BnaC03.MYB56被验证为BnaA09.LEC1.1的转录激活剂.
结论:
- BnaC03.MYB56,BnaA10.AGL15,BnaC04.VAL1和BnaA10.MYB56与WRI1和LAFL共同表达,形成了种子发育和油脂生物合成的模块.
- MYB56积极调节种子油的积累,在Arabidopsis突变物中得到验证.
- 为eQEI识别和洞察种子油积累监管提供了一个框架.
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