性和pH影响了具有不同纳米结构的抗菌性脂的自我组装
Anindyasundar Adak1, Valeria Castelletto1, Bruno Mendes2
1School of Chemistry, Pharmacy and Food Biosciences, University of Reading, Whiteknights, Reading RG6 6AD, U.K.
ACS applied bio materials
|July 23, 2024
概括
性和pH显著影响脂化的自我组装方式. 同体基质形成纳米纤维,而异体基质形成纳米/纳米管,表明具有抗菌性质的pH响应和基质依赖的自我组装.
科学领域:
- 生物分子的自我组装.
- 超分子化学 超分子化学
- 酸科学是一种科学.
背景情况:
- 奇拉性是生物分子自我组装的基础.
- 活体内的脂化驱动自我组装并增强生物活性.
- 对于设计功能生物材料来说,了解体对体自我组合的性影响至关重要.
研究的目的:
- 设计和研究pH响应的,依赖的脂质三的自组合.
- 探索同型性与异型性对自组装结构的影响.
- 评估设计的脂的生物活性和细胞兼容性.
主要方法:
- 合成同性 (l-异构体) 和异性 (d-异构体) 脂化三.
- 使用光谱学,冷传输电子显微镜 (cryo-TEM) 和小角度X射线散射 (SAXS) 进行自我组装的表征.
- 评估pH依赖的结构转变和抗菌活性 (MIC,MBC测定,活/死染色).
主要成果:
- 脂质三在酸性pH下表现出pH响应的自我组装成微粒.
- 在基本的pH值下,同体性脂会形成纳米纤维,而异体性脂会形成纳米和纳米管.
- 证实了pH诱导的转换为β叶结构,并观察到具有良好的细胞相容性的有前途的抗菌活性.
结论:
- 性和pH值是控制脂化三的自我组装行为的关键因素.
- 该研究表明,根据性和pH值,可调节的自我组装成各种纳米结构 (,纳米纤维,纳米带,纳米管) .
- 设计的脂显示出具有抗微生物特性和良好的细胞相容性的生物活性剂的潜力.
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