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脱细胞化树支架的三维孔隙结构调节了细胞培养肉的肌体差异化
Ziying Chen1, Wanli Xiong1, Yuzhe Guo1
1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, P. R. China.
Journal of food science
|July 23, 2024
概括
有纤维孔的食用植物支架增强了细胞培养肉类的生产. 与蜂巢结构相比,大的支架促进了较好的肌肉细胞生长和分化,改善了肉质.
科学领域:
- 生物材料科学 生物材料科学
- 细胞生物学 细胞生物学
- 食品科学 食品科学 食品科学
背景情况:
- 脱细胞化的植物支架为可食细胞培养的肉支架提供了天然结构.
- 脚手架毛孔结构的变化会影响骨肌肉细胞的分化.
研究的目的:
- 为了研究脱细胞化树支架的不同3D孔隙结构如何影响C2C12细胞增殖和肌性分化.
- 为了评估脚手架结构对细胞培养肉质量的影响.
主要方法:
- 的组织被脱细胞化和改造,保留纤维或蜂孔孔结构.
- 在这些支架上培养了C2C12细胞,以评估增殖和分化.
- 由此产生的基于细胞的肉类被分析了质量属性,如蛋白质含量.
主要成果:
- 纤维孔结构促进了平行细胞对齐,而蜂结构导致了圆形细胞连接.
- 纤维性支架导致多核性髓管的增强形成和更高的肌性基因/蛋白质表达.
- 在纤维支架上培养的基于细胞的肉类呈现出更高的总蛋白质含量.
结论:
- 带有纤维孔结构的脱细胞化植物支架对于骨肌细胞的肌体差异化更有效.
- 这一发现支持为细胞培养肉类生产开发优化的食用支架.
- 了解3D毛孔结构的作用对于稳定培养肉类应用中的肌原分化至关重要.
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