产生物质的微生物的定向进化
Julie M Laurent1, Ankit Jain2, Anton Kan1
1Department of Materials, Complex Materials, ETH Zürich, Zürich 8093, Switzerland.
概括
研究人员开发了一个高通量平台,以快速进化微生物,以提高材料生产. 这种方法确定了纤维素生产和细菌中的蛋白质循环之间的新型遗传联系,为可持续制造铺平了道路.
科学领域:
- 微生物学 微生物学
- 合成生物学 合成生物学
- 生物技术是生物技术.
背景情况:
- 微生物自然地以可持续的方式生产材料.
- 原生微生物的生长通常是缓慢的.
- 工程微生物需要对基因型-表型链接的知识.
研究的目的:
- 开发一个高通量定向进化平台.
- 提高微生物适应材料生产的能力.
- 确定改善材料合成的遗传途径.
主要方法:
- 利用以滴滴为基础的微流体平台进行定向进化.
- 向一个由4万个随机突变的Komagataeibacter sucrofermentans*群体施加选择压力.
- 测序进化菌株以识别遗传变化.
主要成果:
- 成功地演变为 *Komagataeibacter sucrofermentans* 导致纤维素过度生产.
- 确定了纤维素生产与蛋白酶复合体基因之间的新联系.
- 展示了平台提高微生物健康和发现基因型-表型关系的能力.
结论:
- 高通量定向进化平台对菌株开发有效.
- 这种方法加速了可持续材料制造.
- 发现了微生物物质生产中的意想不到的遗传洞察力.
相关概念视频
Conditions on Early Earth
91.3K
Around 4 billion years ago, oceans began to condense on earth while volcanic eruptions released nitrogen, carbon dioxide, methane, ammonia, and hydrogen into the primordial atmosphere. However, organisms with the characteristics of life were not initially present on earth. Scientists have used experimentation to determine how organisms evolved that could grow, reproduce, and maintain an internal environment.
91.3K
The Evidence for Evolution
42.6K
Genetic variations accumulating within populations over generations give rise to biological evolution. Evolutionary changes can result in the formation of novel varieties and entire new species. These changes are responsible for the diverse forms of life inhabiting the planet. The evidence for evolution suggests that all living organisms descended from common ancestors.
42.6K
Eukaryotic Evolution
33.3K
The endosymbiont theory is the most widely accepted theory of eukaryotic evolution; however, its progression is still somewhat debated. According to the nucleus-first hypothesis, the ancestral prokaryote first evolved a membrane to enclose DNA and form the nucleus. Conversely, the mitochondria-first hypothesis suggests that the nucleus was formed after endosymbiosis of mitochondria.
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
Contrary to the endosymbiont theory, the eukaryote-first hypothesis proposes that the simpler prokaryotic and...
33.3K
Genome Size and the Evolution of New Genes
7.9K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
7.9K
Gene Evolution - Fast or Slow?
7.1K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.1K
What is Evolutionary History?
36.4K
Scientists record evolutionary history by analyzing fossil, morphological, and genetic data. The fossil record documents the history of life on Earth and provides evidence for evolution. However, both fossil and living organisms offer evidence that outlines Earth’s evolutionary history.
36.4K


