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Updated: Jun 19, 2025

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
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Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Published on: April 2, 2016

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使用CTLR-Seq解决22q11.2删除揭示了染色体重排机制和断点的个体差异

Bo Zhou1,2, Carolin Purmann1,2,3, Hanmin Guo1,2,3,4,5

  • 1Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA 94305.

Proceedings of the National Academy of Sciences of the United States of America
|July 23, 2024
PubMed
概括

我们开发了CRISPR/Cas9针对性的长读序列 (CTLR-Seq) 来分析复杂的基因组区域. 这种方法解决了22q11.2删除综合征中的重排,揭示了转子子驱动的重组和细胞类型特定的DNA甲基化模式.

关键词:
染色体相互作用的相互作用副本号码的变化 副本号的变化基因组重复的重复微切除综合征的发生.有针对性的基因组组装.

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