介绍了一种基于桑德尔-科尔托夫反应的微板上的唾液测定谱光计方法
Adrijana Oblak1,2, Jernej Imperl3, Mitja Kolar3
1Division of Nuclear Medicine, University Medical Centre Ljubljana, Ljubljana, Slovenia.
Radiology and oncology
|July 23, 2024
概括
一种新的,简单的方法准确地测量了唾液度 (SLIC),这对于评估甲状腺激素合成至关重要. 这种可靠的技术为监测状态提供了一个易于使用的替代方案.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 内分泌学 在内分泌学.
背景情况:
- 对于甲状腺激素的产生至关重要.
- 准确评估供应是非常重要的.
- 唾液腺是分泌的一个途径.
研究的目的:
- 开发和验证一种新的,简单的方法来确定唾液度 (SLIC).
- 建立SLIC作为对状态的可靠标记.
主要方法:
- 利用桑德尔-科尔托夫反应与基于微板的,自制试剂.
- 使用水基标准 (WBS) 验证了该方法,并将结果与诱导合等离子体质谱法 (ICP-MS) 进行了比较.
- 优化过氧化硫酸 (AP) 度和评估样本稀释因子和潜在干扰.
主要成果:
- 基于水的标准 (WBS) 提供了比基于人工唾液的标准 (ASS) 更可靠的结果.
- 该方法表明空白限值 (LoB) 为6.5μg/L,检测限值 (LoD) 为12.0μg/L,分析范围为12-400μg/L.
- 在大多数度下,观察到高精度,测试内和测试间的不精确度低于10%,该方法与ICP-MS (斯皮尔曼系数0.989) 非常一致.
结论:
- 开发的SLIC测定方法准确,精确,易于使用.
- 新的SLIC方法与ICP-MS非常一致,证实了其可靠性.
- 这种方法为通过唾液分析评估状态提供了有价值的工具.
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