缺氧后调节减轻了缺氧引起的炎症和内皮屏障功能障碍
Jiaxing Ma1, Yinhua Zhao1, Yue Cui1
1Plastic and Reconstructive Surgery, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
The Journal of surgical research
|July 23, 2024
概括
缺氧后调节 (HPC) 保护人类皮肤内皮细胞免受缺氧再氧化 (HR) 损伤. HPC抑制P38 MAPK,保持内皮屏障并减少炎症.
科学领域:
- 细胞生物学 细胞生物学
- 身体生理学 身体生理学
- 生物化学 生化学
背景情况:
- 缺氧复氧化 (HR) 可以导致内皮屏障功能障碍和炎症.
- 激活P38基激活蛋白激酶 (P38 MAPK) 有助于HR期间内皮屏障的分解.
- 缺氧后调节 (HPC) 对人体皮肤微血管内皮细胞中P38 MAPK的具体影响尚不清楚.
研究的目的:
- 研究HPC在人类皮肤微血管内皮细胞中对HR诱导的内皮损伤的保护功能.
- 确定P38 MAPK信号在HPC的保护作用中的作用.
- 检查HPC对内皮屏障完整性和炎症标志物的影响.
主要方法:
- 人体皮肤微血管内皮细胞接受了缺氧复氧化 (HR) 或缺氧后条件化 (HPC).
- 评估了细胞活性,炎症性细胞因子水平 (IL-6,IL-8,IL-1β) 和内皮屏障的透性.
- 分析了P38 MAPK激活 (P-P38 MAPK),P38 MAPK表达以及克劳丁-5水平和局部化,使用西斑和免疫光学.
主要成果:
- HR导致内皮膜的透性增加,炎症和P-P38 MAPK水平增加.
- HPC治疗显著减少了细胞损伤,并保持了内皮屏障的完整性.
- HPC抑制了P-P38 MAPK,增加了克劳丁-5的表达,并促进了其在细胞膜上的持续定位.
结论:
- HPC 显示出对低氧化重氧化诱导的内皮壁障碍功能障碍的保护作用.
- 该机制涉及P38 MAPK信号通路的抑制.
- 通过调节Claudin-5的表达和局部化,HPC保留了内皮屏障的完整性.
相关概念视频
01:23Hypoxia
994
Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
994
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
01:20Chronic Obstructive Pulmonary Disease-II: Pathophysiology
2.7K
Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Chronic Inflammation
2.7K
01:28Inflammatory Response
1.9K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
1.9K