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相关概念视频

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.

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相关实验视频

Updated: May 8, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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4D单粒子跟踪与异步读出单光子雪崩二极管阵列探测器阵列探测器

Andrea Bucci1,2, Giorgio Tortarolo1,3, Marcus Oliver Held1

  • 1Molecular Microscopy and Spectroscopy, Istituto Italiano di Tecnologia, Genoa, Italy.

Nature communications
|July 23, 2024
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种增强的共聚焦显微镜,用于精确的单粒子跟踪和光寿命测量. 新系统改善了生物研究的时空分辨率.

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Studying Soft-matter and Biological Systems over a Wide Length-scale from Nanometer and Micrometer Sizes at the Small-angle Neutron Diffractometer KWS-2
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科学领域:

  • 生物物理学的生物物理.
  • 显微镜的使用方法
  • 细胞生物学 细胞生物学

背景情况:

  • 单粒子追踪 (SPT) 对于理解生物粒子动态至关重要.
  • 现有的 SPT 方法在解决方案,复杂性和数据采集方面面临着权衡.
  • 需要先进的显微镜技术,提高时空精度.

研究的目的:

  • 为了增强共聚焦激光扫描显微镜,以实现卓越的单粒子跟踪.
  • 整合光寿命测量与实时粒子定位.
  • 克服生物研究当前的SPT技术的局限性.

主要方法:

  • 增强了对焦激光扫描显微镜与异步单光子雪崩二极管阵列探测器.
  • 实现了实时反系统,以保持粒子在激发体积内的中心.
  • 结合单粒子跟踪与光寿命测量,使用光子检测.

主要成果:

  • 通过100个光子实现了40nm横向和60nm轴向定位精度.
  • 启用分毫秒时间采样,用于实时跟踪,线下微秒尺度的精制.
  • 在活细胞中成功跟踪光珠和溶解体,测量了膜蛋白光寿命.

结论:

  • 增强的显微镜系统为SPT的时空分辨率提供了显著的改进.
  • 相对追踪和光寿命测量提供了更丰富的生物学见解.
  • 这种先进的显微镜方法有望促进新的相关成像和跟踪研究.