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在疑似中枢神经系统感染的病原体检测中,基因组下一代测序的临床应用和评估

Lei Yuan¹, Xin Yu Zhu¹, Lan Min Lai¹

¹Department of Clinical Laboratory, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, People's Republic of China.

Scientific reports

|July 23, 2024

概括

与传统方法相比,元基因组下一代测序 (mNGS) 为中枢神经系统感染 (CNSIs) 提供了优越的病原体检测. 这种先进的技术提高了诊断准确度,有助于改善这些复杂疾病的患者结果.

科学领域:

神经学神经学
传染性疾病传染性疾病
基因组学就是基因组学.

背景情况:

由于复杂的病原体和快速进展,中枢神经系统感染 (CNSIs) 存在重大诊断挑战.
高死亡率和残疾率强调需要准确和及时诊断CNSIs.

研究的目的:

评估元基因组下一代测序 (mNGS) 对于诊断中枢神经系统疾病的临床实用性.
将mNGS的诊断性能与传统方法 (如培养和涂抹) 进行比较.
为了确定影响mNGS结果的因素在CNSI患者中.

主要方法:

一项追溯性研究涉及111名疑似中枢神经神经系统感染的患者.
将mNGS结果与传统诊断技术进行比较.
统计分析,包括奇平方测试,以评估诊断准确性和显著性 (p < 0.05).

主要成果:

与培养 (26.5%,p < 0.0001) 相比,mNGS在CNSIs患者中显示出更高的病原体检测率 (68.7%).
mNGS的灵敏度和特异性分别为68.7%和82.9%.
针对病毒性脑炎/脑膜炎的最佳表现观察到的是特定物种的读数≥2 (AUC0.758).
mNGS显示细菌中枢神经神经系统有明显脑脊髓液异常的阳性率增加.

结论:

传统的微生物学测试往往不足以检测所有神经侵入性病原体.
mNGS对CNSIs具有令人满意的诊断性能,在总体检测率方面超过了文化.
mNGS是改进中枢神经系统感染诊断的宝贵工具.

关键词:

申请申请表申请表中枢神经系统感染中枢神经系统感染诊断诊断诊断诊断诊断下一代元基因组测序的测序.病原体是一种病原体.

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