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乳牛母乳养引起的压力诱导的表观遗传效应:一种甲基化网络方法

Adrián López-Catalina^1,2,3, Antonio Reverter³, Pamela A Alexandre³

¹Departamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), CSIC, Crta. de la Coruña km 7.5, Madrid, Spain.

|July 24, 2024

概括

母乳哺乳状态影响小牛的表观遗传学. 与哺乳母牛出生的小牛相比,非哺乳母牛产生的小牛显示出不同的DNA甲基化模式,影响了发育.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
动物科学动物科学
基因组学就是基因组学.

背景情况:

表观遗传标记不是通过孟德尔定律遗传的,可以受到环境因素的影响.
哺乳母牛在孕期早期经历负能量平衡,可能会影响胚胎发育.
乳腺组织和正在发育的胚胎之间的营养竞争是哺乳母牛的一个问题.

研究的目的:

为了研究非哺乳母牛和哺乳母牛产生的小牛的甲基化概况的差异.
探索母体表型与后代表观基因组之间的相关性.
为了确定与母乳哺乳状态和后代特征相关的特定表观遗传变化.

主要方法:

全基因组双硫酸盐测序以确定差异甲基化细胞因子 (DMCs) 和区域 (DMRs).
构建一个甲基化网络来分析母体表型和小牛表观基因组之间的相关性.
基因特异性分析以确定已识别的表观遗传标记的功能关联.

主要成果:

在两组小牛之间,确定了50,277个DMC和2,281个DMR.
一个甲基化网络揭示了265个与母体表型相关的区域.
DMC和DMR与母牛的哺乳状态以及小牛对牛奶特征 (EBV) 的基因组预测有关.

结论:

不哺乳母孕育的小牛与哺乳母母孕育的小牛相比,表现出不同的甲基化概况.
表观遗传差异与母亲的哺乳期有关,并影响后代的发育和遗传潜力.
这些发现支持母亲环境在塑造后代表观基因组的作用,这超出了遗传遗传的范围.

关键词:

甲基化网络的构成.牛奶牛表观遗传学牛奶牛表观遗传学不同的甲基化.甲基化生物标志物甲基化生物标志物全基因组甲基化.

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