[雌激素受体Esr1诱导Trachemys scripta的早期卵巢分化]
Jinhong Guo1,2, Hongzhe Yang1,3, Xiang Shi2
1Institute of Animal Sex and Development, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China.
概括
乌胚胎中的雌激素受体1 (Esr1) 激活会触发雌性生殖腺的发育,即使在产生雄性的温度下也是如此. 这表明Esr1调解了雌激素在乌早期卵巢分化中的作用.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 内分泌学 在内分泌学.
背景情况:
- 雌激素受体 (Esr1,Esr2,Gper1) 在脊椎动物的繁殖发育中至关重要.
- 这些受体在的早期性腺分化中的特定作用在很大程度上仍未被探索.
- 在像Trachemys scripta这样的海中,取决于温度的性别确定涉及复杂的荷尔蒙信号.
研究的目的:
- 研究Esr1,Esr2和Gper1在Trachemys scripta*早期胚胎性腺区分中的作用.
- 为了确定激活特定的雌激素受体是否可以覆盖基于温度的性别确定线索.
主要方法:
- 研究了胚胎生殖腺中*esr1*,*esr2*和*gper1*基因的表达模式.
- 在男性生产温度 (MPT) 处向胚胎注射特定激素 (PPT为Esr1,WAY 200070为Esr2,G-1为Gper1).
- 分析了生殖腺形态,生殖细胞分布,以及关键的性别分化基因 (*dmrt1*, *amh*, *sox9*, *foxl2*, *cyp19a1*) 和蛋白质 (Amh, Sox9, Foxl2, Aromatase) 的表达.
主要成果:
- 在关键分化阶段和雌性生产温度 (FPT) 时,Esr1表达最高.
- 在MPT的PPT治疗诱导了性腺的显著女性化,包括形态,生殖细胞模式和基因表达 (男性标记物的下调,女性标记物的上调).
- WAY 200070和G-1治疗没有诱导性别逆转; 性腺发育为丸与男性基因表达模式.
结论:
- 仅仅激活Esr1就足以在Trachemys scripta*中启动早期的卵巢分化.
- 在这种物种中,雌激素可能主要通过Esr1通路诱导早期的卵巢分化.
- 这些发现提供了对雌激素介导性决定和的分化机制的关键见解.
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