Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Atomic force microscopy for quantitative nanomechanical characterization of immune cells: techniques and applications in allergic responses.

Nanotechnology·2026

Same author

AFM-based nanomechanical evaluation of human umbilical cord mesenchymal stem cells for alcohol-associated liver injury.

Analytical methods : advancing methods and applications·2026

Same author

Multi-Objective Optimization of Damage Volume and CO<sub>2</sub> Consumption for High-Pressure Liquid CO<sub>2</sub> Jet Impact on Hydroxyl-Terminated Polybutadiene Propellant.

Materials (Basel, Switzerland)·2026

Same author

Parameter-Free Attention Super-Resolution Network for Accelerated AFM Biological Imaging.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Deep Learning-Assisted Nanomechanical Evaluation of Replicative Senescence in Human Umbilical Cord Mesenchymal Stem Cells.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Enzymatic Redox Gating Directs Oxidative Divergence in Acyclic Peroxides Biosynthesis.

Angewandte Chemie (International ed. in English)·2026

Search research articles

相关实验视频

Updated: Jun 19, 2025

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

用原子力显微镜测量IgE分子的pH诱导的变化.

Jing Hu^1,2,3,4, Zuobin Wang^2,3,4,5, Dayong Jiang¹

¹School of Materials Science and Engineering, Changchun University of Science and Technology, Changchun, China.

Microscopy research and technique

|July 24, 2024

概括

蛋白质结构对其环境非常敏感. 这项研究揭示了pH值如何影响免疫球蛋白E (IgE) 分子的形状,大小和聚合,为不同条件下的蛋白质行为提供了洞察力.

关键词:

的IgE分子.图像检测检测检测图像检测检测检测它们的pH值是pH值.超结构的超结构.

更多相关视频

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Published on: March 6, 2017

相关实验视频

Last Updated: Jun 19, 2025

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Published on: March 6, 2017

科学领域:

生物化学生物化学
生物物理学的生物物理.
蛋白质科学蛋白质科学

背景情况:

蛋白质的形状和动态受其周围环境的影响.
了解不同条件下的蛋白质行为对于生物和医学研究至关重要.

研究的目的:

研究pH对免疫球蛋白E (IgE) 分子超结构的影响.
探索pH和IgE形态,高度和面积之间的关系.

主要方法:

利用原子力显微镜 (AFM) 来对IgE分子进行成像.
在各种pH条件下化IgE分子以观察结构变化.

主要成果:

IgE分子形态,高度和面积对pH非常敏感.
酸性pH诱导小尺寸的圆形IgE结构.
性pH促进了自我组装成大型,类似花朵的IgE聚合物.

结论:

IgE分子结构和聚合显著依赖pH值.
这项研究为进一步研究IgE在各种物理和化学因素下的行为提供了基础.
该AFM方法可以适应研究由其他环境变量诱导的IgE修饰.