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谷氨胺通过提高细胞内pH值来增强在甲氨酸半饥饿下肺炎球菌的生长

Chengwang Zhang¹, Juncheng Liu¹, Xiaohui Liu^2,3

¹Department of Basic Medical Science, School of Medicine, Lishui University, Lishui, Zhejiang, China.

Frontiers in microbiology

|July 24, 2024

概括

细菌通过半饥饿适应营养缺乏,其中细胞内酸性抑制生长. 谷氨胺补充剂通过增加细胞内pH值来恢复生长,为抗病原体提供潜在的药物标.

科学领域:

微生物学微生物学
细菌生理学细菌生理学
病变的发生和发病.

背景情况:

细菌在自然环境中面临营养限制,需要适应生存和繁殖的策略.
了解细菌对营养缺乏的反应对于控制病原细菌引起的传染病至关重要.

研究的目的:

调查营养限制期间的细菌适应机制,特别是Streptococcus pneumoniae中 metionin半饥饿.
阐明细胞内pH值和营养物质可用性的作用,以调节细菌在不理想条件下的生长.

主要方法:

细菌生长曲线,细胞内pH值,氨基酸含量,基因转录,蛋白质表达和酶活性的分析.
操纵营养物质的可用性 (甲氨酸,谷氨酸) 以评估对细菌生理和生长的影响.

主要成果:

在Streptococcus pneumoniae中,氨酸的半饥饿导致增长减弱,主要是由于细胞内酸化,而不是氨酸缺乏.
补充谷氨酸恢复了细菌的生长,通过将细胞内pH值提高到最佳水平 (~7.6),而不会改变细胞内 metionin水平.
发现细胞内酸化会降低翻译水平,在pH值通过谷氨正常化后恢复.

结论:

细菌对半饥饿的适应涉及复杂的pH调节,其中谷氨酸作为恢复生长的关键因素.
通过细胞内pH调整恢复生长的已识别的机制提出了一个新的细菌适应策略.
针对这种pH介导的适应途径可以在营养有限的条件下提供针对致病细菌的新疗法策略.

关键词:

肺炎链球菌的肺炎链球菌.谷氨胺是一种胺.增长的增长增长的增长增长的增长.细胞内部的pH值甲氨酸的使用方法在半饥饿的状态下.

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