新疆棕牛的外周血液中的DNA甲基化模式,体细胞数量可变
Dan Wang1, Shengchao Ma1, Mengjie Yan1
1College of Animal Science, Xinjiang Agricultural University, Urumqi, China.
Frontiers in genetics
|July 24, 2024
概括
新疆棕牛的体细胞计数 (SCC) 揭示了乳腺炎耐药性的表观遗传生物标志物. 不同甲基化的基因TRAPPC9和CD4在乳腺炎中表达变化,有助于基因改进.
科学领域:
- 动物遗传学和动物基因组学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 兽医免疫学 兽医免疫学
背景情况:
- 体细胞计数 (SCC) 是乳牛群改善的关键指标,与乳腺炎耐药性有关.
- 之前的研究已经确定了与乳腺炎耐药性相关的基因,但表观遗传机制仍然不太了解.
研究的目的:
- 调查新疆棕色牛的全基因组DNA甲基化模式,具有不同的SCC.
- 为了确定这种牛品种中乳腺炎耐药性的表观遗传生物标志物.
主要方法:
- 使用MeDIP-seq对具有不同SCCs的牛血液样本进行全基因组DNA甲基化分析.
- 使用双硫酸基因组测序对TRAPPC9和CD4基因中CpG岛屿的微分甲基化分析.
- 通过RT-qPCR和体外细胞培养模型进行基因表达分析.
主要成果:
- 确定了1934个与乳腺炎耐药性相关的差异表达基因 (DEGs).
- 在乳腺炎病例中,TRAPPC9的甲基化水平较高,而CD4的甲基化水平较低.
- 在受乳腺炎影响的牛群中,TRAPPC9和CD4基因表达显著减少.
结论:
- 对TRAPPC9和CD4的表观遗传变化与新疆棕色牛的乳腺炎易感性有关.
- 这些发现为乳腺炎治疗,育种和基因改进策略提供了理论基础.
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