用Euphorbia tirucalli提取物加载的聚合物纳米颗粒的体外抗真菌活性
M S Heya1, M J Verde-Star2, C Rivas-Morales2
1Universidad Autónoma de Nuevo León, Faculty of Public Health and Nutrition, San Nicolas de los Garza, Nuevo León, México.
概括
药用植物提取物显示出抗真菌潜力,但溶解性差限制了有效性. 优花提取物的纳米颗粒配方显著增强了对Trichophyton物种的抗真菌活性.
科学领域:
- 药理学和植物化学
- 纳米技术和药物输送
背景情况:
- 药用植物提供治疗潜力,但面临着诸如低生物可用性和可溶性等局限性.
- 纳米载体系统代表了先进的药物输送解决方案,以克服传统配方的挑战.
研究的目的:
- 评估Euphorbia tirucalli的甲醇提取物在聚合物纳米颗粒中制成的体外抗真菌活性.
- 评估纳米载体配方对Trichophyton rubrum和T. interdigitalis临床分离物的疗效的影响.
主要方法:
- 装有植物提取物 (NP-Ext) 的纳米粒子是使用纳米沉制备的.
- 通过微稀释试验确定了抗真菌活性,计算了最小抑制度 (MIC).
- 纳米粒子的物理化学特征和提取物封装效率使用FTIR光谱学进行了分析.
主要成果:
- 聚合物纳米颗粒表现出一致的物理化学特性 (大小~91.9nm,PDI~0.15,Z电位~-6.05).
- 封装效率和负载被确定为分别为2.95%和22.15%.
- NP-Ext显著改善了抗真菌活性,将MIC值从125μg/mL (原始提取物) 降低到55.55μg/mL和0.1μg/mL对T. rubrum和T. interdigitalis.
结论:
- 配方提取物的增强抗真菌功效归因于纳米载体的物理化学特性.
- 纳米粒子配方成功提高了Euphorbia tirucalli提取物对皮肤菌感染的治疗潜力.
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