相关实验视频

Updated: Jun 19, 2025

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

阿雷科林通过上调IL-4的表达来促进纤维细胞激活和M2-巨细胞两极分化

Zhenying Yuan¹, Ming Li²

¹Changsha Stomatological Hospital, Changsha 410000, China.

Archives of oral biology

|July 24, 2024

概括

阿拉科林增加了互乐金-4 (IL-4) 的表达,通过激活纤维细胞和M2-巨细胞来促进口腔下纤维化 (OSF). 抑制IL-4可以降低这些效应,突出IL-4的作用.

科学领域:

口腔病理学口腔病理学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

口腔膜纤维化 (OSF) 是一种潜在的恶性疾病,与阿拉卡坚果的有关.
目前尚不完全理解OSF病原发生的精确分子机制.

研究的目的:

研究阿雷科林对口腔下纤维化 (OSF) 的生物学影响.
阐明介素-4 (IL-4) 在阿雷科林诱导的OSF发育中的作用.

主要方法:

使用GEO数据集对OSF和正常口腔组织的基因表达分析.
实时PCR和免疫组织化学检测IL-4和M2巨细胞标记物.
通过ELISA,CCK-8和transwell测定来评估纤维细胞活性.
通过IL-4倒置和流动细胞计量来分析巨细胞极化.

主要成果:

在口腔纤维细胞 (HOF) 中,阿雷科林上调调节IL-4基因和蛋白质表达.
阿雷科林促进了HOF细胞的增殖和迁移,效应被IL-4敲击减弱.
重组IL-4增加了M2巨细胞的比例,OSF组织中ARG1的表达升高.

结论:

阿雷科林通过上调IL-4的调节驱动OSF的进展.
纤维细胞的IL-4激活和M2-巨细胞极化是阿雷科林诱导的OSF中的关键机制.

关键词:

纤维母细胞细胞是如何形成的在M1-巨细胞中.在M2巨中,巨是M2-巨.巨细胞是一个巨细胞.在口腔下粘膜纤维化.

更多相关视频

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Published on: May 5, 2023

相关概念视频

Introduction to Fibroblasts

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...