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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Nucleic Acids02:43

Nucleic Acids

44.0K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
44.0K
RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Improving Translational Accuracy02:07

Improving Translational Accuracy

9.8K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
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Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
Nucleic Acid Structure01:25

Nucleic Acid Structure

6.1K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
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相关实验视频

Updated: Jun 19, 2025

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
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在破译RNA-RNA相互作用的技术进步.

Rong Ye1, Hailian Zhao2, Xi Wang3

  • 1Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

Molecular cell
|July 24, 2024
PubMed
概括

高通量技术在全球范围内绘制RNA-RNA相互作用 (RRI),揭示它们在多种生物体的基因调节中的关键作用. 本综述详细介绍了了解RRI的方法,见解和未来挑战.

关键词:
在RIC-seqq.在RNA-RNA相互作用中.这是一种RNA结合蛋白.亲近性结合 (proximity ligation) 是一种接近性结合.

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Nanomanipulation of Single RNA Molecules by Optical Tweezers
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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
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An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

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相关实验视频

Last Updated: Jun 19, 2025

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Nanomanipulation of Single RNA Molecules by Optical Tweezers
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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 通过复杂的高阶结构和基质结合,RNA分子在生物过程中发挥着关键作用.
  • 了解RNA-RNA相互作用 (RRI) 对于阐明RNA功能,结合特异性和调节机制至关重要,特别是对于非编码RNA.
  • 全球RRI映射为复杂的基因调节提供了宝贵的见解.

研究的目的:

  • 审查和总结用于全球RNA-RNA相互作用 (RRI) 地图的最先进的高通量技术.
  • 分析各种RRI映射技术的关键概念,优点和缺点.
  • 突出通过RRI绘图实现的新生物学发现,并讨论未来的研究方向.

主要方法:

  • 专注于用于绘制RNA-RNA相互作用的高通量实验和计算技术.
  • 不同RRI映射方法的比较分析.
  • 用RRI映射来发现生物学见解的研究的文献综述.

主要成果:

  • 介绍了全球RRI绘制先进技术的全面概述.
  • 总结了各种RRI映射方法的关键概念,优点和缺点.
  • 突出了从各种生物体的RRI映射中获得的新生物学见解.

结论:

  • 高通量RRI映射技术对于了解RNA功能和基因调节至关重要.
  • 这些方法的持续开发和应用将加深我们对RRI关键角色的认识.
  • 未来的挑战在于完全破译RRI调解的复杂监管网络,这些监管网络遍及生活的各个领域.