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Updated: Jun 19, 2025

A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis

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Published on: March 15, 2016

在软体动物模型物种Lymnaea stagnalis中对corazonin信号的表征

István Fodor¹, Tomohiro Osugi², Shin Matsubara²

¹Ecophysiological and Environmental Toxicological Research Group, HUN-REN Balaton Limnological Research Institute, Tihany 8237, Hungary.

General and comparative endocrinology

|July 24, 2024

概括

这项研究将软体动物性腺激素释放激素 (GnRH) 重归类为心肌素 (CRZ). 研究人员描述了Lymnaea stagnalis中的corazonin信号系统,证实了CRZ.

科学领域:

神经内分泌学神经内分泌学
比较生理学比较生理学
分子进化分子进化

背景情况:

最近的证据表明,神经原先被称为软性腺激素释放激素 (GnRH) 是多功能性的,应该被归类为corazonin (CRZ).
在文献中仍然存在错误分类,将脊椎动物的GnRH功能归因于软体动物的CRZ.
澄清GnRH超级家族的命名和功能对于理解神经的演变至关重要.

研究的目的:

为了支持最近将软体动物GnRH重新归类为CRZ.
为了描述 Lymnaea stagnalis. 软体的心脏素 (CRZ) 信号系统.
为了提供对GnRH神经超级家族内的进化关系的见解.

主要方法:

对Lymnaea转录组进行同质性搜索,以识别CRZ受体类序列 (Lym-CRZR).
分子系遗传学分析以确定受体聚类.
HEK293细胞转移以评估受体定位,血功能和信号通路 (Ca2+调动,cAMP产生).
对Lym-CRZR的组织分布分析.

主要成果:

一个类似CRZ受体的序列 (Lym-CRZR) 被确定并与软体动物CRZR分类.
莱姆-CRZR作为膜受体起作用,在感染过的HEK293细胞中定位在血膜上.
软体动物神经Lym-CRZ刺激了剂量依赖的细胞内Ca2+调动,但不是通过Lym-CRZR产生的cAMP.
在Lymnaea stagnalis中,Lym-CRZR表现出广泛的组织分布.

结论:

这些发现支持Lym-CRZ的多功能性质,并将其归类为corazonin (CRZ).
这项研究提供了进一步的证据来重新分类以前识别的软体动物GnRH作为CRZ.
对Lym-CRZ信号系统的表征加深了我们对GnRH超级家族进化的理解.

关键词:

在Corazonin里面释放性荷尔蒙释放性荷尔蒙 (Gonadotropin) 是一种释放性荷尔蒙.林 (Lymnaea stagnalis) 是一种常年存在的植物.软体动物软体动物接收器接收器接收器

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