来自克拉姆阳性细菌Clostridium ventriculi的两种葡萄糖转移酶2基因编码 (1,3;1,4) -β-D-葡萄糖合成酶
Shu-Chieh Chang1, Rebecka Karmakar Saldivar1, Mu-Rong Kao2
1Division of Glycoscience, Department of Chemistry, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH Royal Institute of Technology, AlbaNova University Centre, Stockholm, SE10691, Sweden; School of Pharmacy, College of Pharmacy, Taipei Medical University, 250 Wuxing Street, Taipei 11031, Taiwan.
Carbohydrate polymers
|July 24, 2024
概括
菌在它的外聚糖中产生 (1,3;1,4) -β-D-葡萄糖,而不是纤维素. 基因组和功能研究确定了两个基因,CvGT2-1和CvGT2-2,负责合成这些葡萄糖.
科学领域:
- 微生物学 微生物学
- 细菌生理学 细菌生理学
- 葡萄糖生物学 葡萄糖生物学
背景情况:
- 外聚糖 (EPS) 是具有多样性结构和功能的关键细菌成分.
- 最近发现,罗姆布西亚 (Romboutsia ilealis) 能够产生 (1,3;1,4) -β-D-葡萄糖.
- 从历史上看,Clostridium ventriculi被认为在其EPS中含有大量的纤维素.
研究的目的:
- 为了研究Clostridium ventriculi中的外聚糖的组成.
- 为了确定C. ventriculi EPS.中存在的特定葡萄糖.
- 确定C. ventriculi.中的EPS合成的遗传基础.
主要方法:
- 使用化酶对C. ventriculi外聚糖的酶性水解.
- 释放的寡糖的分析.
- 基因组分析以识别潜在的葡萄糖转移酶基因.
- 在Saccharomyces cerevisiae中的功能获取实验.
主要成果:
- 酶处理释放了寡糖,证实了C. ventriculi EPS.中存在 (1,3;1,4) -β-D-葡萄糖的存在.
- 在C. ventriculi EPS中先前假设的纤维素可能被错误识别为 (1,3;1,4) -β-D-glucans.
- 两个基因CvGT2-1和CvGT2-2被确定为编码 (1,3;1,4) -β-D-葡萄糖合成酶的基因.
- 在酵母中的功能表达证实了CvGT2-1和CvGT2-2的酶活性.
结论:
- 克洛斯特里腹膜合成 (1,3;1,4) -β-D-葡萄糖,而不是纤维素,作为主要的外聚糖成分.
- 基因CvGT2-1和CvGT2-2负责C. ventriculi.中的这些 (1,3;1,4) -β-D-葡萄糖的生物合成.
相关概念视频
Oligosaccharide Assembly
2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
2.8K
Glucose Transporters
22.6K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
22.6K
Role of Microtubules in Cell Wall Deposition
2.4K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.4K
Protein Glycosylation
6.9K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
6.9K


