揭示各种直流电能条件对不同多药耐药细菌的有害影响:一项综合性研究
Mamdouh M Shawki1, Hadeel S El-Shall2, Maisa E Moustafa3
1Medical Biophysics Department, Medical Research Institute, Alexandria University, Alexandria, Egypt. mamdouh971@hotmail.com.
Scientific reports
|July 24, 2024
概括
直流 (DC) 电刺激有效地通过破坏细胞结构和降低抗生素耐药性来对抗多药性 (MDR) 微生物. 这种方法在废水净化等应用中显示出作为消毒剂替代品的前景.
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 环境科学 环境科学
背景情况:
- 耐多药 (MDR) 微生物对公共卫生和社会经济构成重大威胁,受污染废物排放加剧了这一威胁.
- 电刺激,特别是直流 (DC),提供了一种潜在的方法来控制微生物的遗传变化,并减少MDR的流行.
研究的目的:
- 研究直流电场在抑制关键医院病原体的生长和活动方面的有效性.
- 为了确定不同电能水平,电压和暴露时间对MDR细菌的影响.
- 评估直流处理作为消毒剂替代品的潜力.
主要方法:
- 研究了直流电场对四种MDR细菌菌株的影响:Pseudomonas aeruginosa,大肠杆菌,金黄色葡萄球菌和Enterococcus faecalis.
- 量化细菌数量,代谢活性,总和细胞外蛋白质含量以及乳酸脱酶活性.
- 分析了使用SEM的活性氧物种 (ROS) 水平,DNA碎片化,等离子体分解性和形态变化.
- 评估了DC治疗对抗生素耐药性概况的影响.
主要成果:
- 电能对细菌生长和新陈代谢产生了剂量依赖的对抗作用,超过了电压或时间单独的影响.
- 暴露于140 J的电能抑制了超过78%的细菌数量 (格拉姆阴性) 和47-73% (格拉姆阳性),与相应的代谢抑制.
- 电流处理诱导了可生存但不可培养 (VBNC) 状态,引起细胞壁和膜损伤,增加ROS,DNA碎片化和降解等质粒.
- SEM显示出显著的形态变形,DC治疗减少了超过64.2%的抗生素耐药性.
结论:
- 电流电刺激是对抗微生物污染,特别是MDR菌株的有效工具.
- 该研究强调了电能诱导VBNC状态并损害细菌完整性的能力.
- 电流处理有效降低了抗生素耐药性,这表明它在牛奶杀菌和废水净化等领域具有作为环保消毒剂的潜力.
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