一种源自病毒体的小干扰RNA准了bHLH转录因子MdPIF1,以调节马家属的安托西亚尼生物合成
Zhenlu Zhang1, Zhao-Yang Li1, Fu-Jun Zhang1,2
1National Key Laboratory of Wheat Improvement, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, China.
Plant, cell & environment
|July 25, 2024
概括
一种新型的病毒源小干扰RNA (vsiR693) 向MdPIF1转录因子,以调节果中的安托西亚宁生物合成. 这一发现揭示了病毒诱导的果症状背后的分子机制.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 果实科学 果实科学
背景情况:
- 果色对果的质量和市场价值至关重要.
- 病毒诱导的斑点症状会对果的颜色产生负面影响.
- 背后的分子机制 viroid 影响水果颜色在很大程度上是未知的.
研究的目的:
- 调查果果果病毒 (ADFVd) 影响果颜色的机制.
- 为了识别特定的小RNA,涉及到病毒诱导的水果颜色变化.
- 阐明向宿主因子在阳素生物合成调节中的作用.
主要方法:
- 5' RLM-RACE用于识别小干扰RNA (siRNA) 目标.
- 人工微RNA短暂表达系统以验证siRNA功能.
- 基因表达分析 (促进体结合和转录试验) 针对MdPIF1和安东素通路基因.
- 在果果中透感染性和突变的ADFVd克隆.
主要成果:
- 鉴定出一种源自病毒的siRNA,VsiR693,它向MdPIF1mRNA.
- MdPIF1通过直接与MdPAL和MdF3H的促进体结合,积极调节氨酸生物合成.
- vsiR693 负面调节了氨酸生物合成,其表达抑制了 MdPIF1 的活性.
- ADFVd感染抑制了水果的颜色,而一个缺乏 vsiR693 生产的突变ADFVd却没有.
结论:
- 病毒衍生的小干扰RNA可以准宿主转录因子来操纵代谢途径.
- vsiR693通过通过MdPIF1.1降低安托氨酸生物合成的调节,在病毒诱导的果点症状中发挥关键作用.
- 这项研究揭示了一种新的病毒-宿主相互作用机制,影响水果质量.
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