在体外检查局部应用益生菌的基因组影响
James V Gruber1, Robert Holtz2, Megan Roach1
1Vantage, Fairfield, New Jersey, USA.
International journal of cosmetic science
|July 25, 2024
概括
这项研究探讨了不同的益生菌细菌,包括Lactobacillus plantarum,如何在重建的人类表皮 (RHE) 模型中影响皮肤基因表达. 这些发现显示每个益生菌的基因调节不同,为皮肤健康的局部益生菌应用提供了洞察力.
科学领域:
- 微生物学 微生物学
- 皮肤病学 皮肤病学
- 基因组学就是基因组学.
背景情况:
- 局部益生菌为皮肤健康提供了潜在的好处.
- 重建的人类表皮 (RHE) 模型是研究皮肤微生物相互作用的宝贵工具.
- 之前的研究已经在局部配方中探索了 Lactobacillus plantarum.
研究的目的:
- 研究各种局部益生菌培养对RHE的影响.
- 建立一种评估益生菌对皮肤基因表达的影响的方法.
- 为了比较不同益生菌物种诱导的基因表达特征.
主要方法:
- 制备了四种局部"只需添加水"益生菌粉末 (Lactobacillus plantarum,Saccharomyces cerevisiae,Streptococcus thermophilus,Lactococcus lactis) 的研究结果.
- 益生菌粉末被转化为无防腐剂的奶油,并应用于RHE组织24小时.
- 用人类基因微阵列进行基因表达分析,重点关注与皮肤相关的基因.
主要成果:
- 植物乳杆菌对4.9%的皮肤相关基因进行了上调调节.
- 在皮肤相关基因中,Saccharomyces cerevisiae调高了7.8%.
- 热球菌 (Streptococcus thermophilus) 的皮肤相关基因上调为6.1%,乳球菌 (Lactococcus lactis) 的皮肤相关基因上调为7.0%.
结论:
- 通过将粉末转化为奶油,成功开发了一种在RHE上检查局部益生菌的方法.
- 不同的益生菌物种诱导RHE.不同的基因表达变化.
- 这种方法有助于研究个人益生菌对皮肤的影响.
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