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激活STING可以提高temozolomide在PTEN中的效率,这些PTEN可以容纳质母细胞瘤细胞

Zafer Yildirim^1,2, Eda Doğan¹, Hale Güler Kara^1,2

¹Department of Medical Biology, Faculty of Medicine, Ege University, İzmir, Turkiye.

Turkish journal of medical sciences

|July 25, 2024

概括

刺痛激动剂通过激活刺痛通路来增强PTEN阳性质母细胞细胞中temozolomide的疗效. PTEN状态预测了对联合治疗的反应,改善了质母细胞瘤治疗结果.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

质母细胞瘤是一种具有不良预后的侵袭性脑瘤.
泰莫佐洛米德是标准化疗,但耐药性很常见.
刺痛激动剂通过I型干扰素诱导调节瘤微环境.

研究的目的:

研究质母细胞瘤细胞系中的STING激活.
确定PTEN蛋白在STING通路激活中的作用.
评估STING激动剂和temozolomide的联合疗效.

主要方法:

用STING激动剂和temozolomide治疗T98G和U118MG质母细胞瘤细胞.
分析了使用qRT-PCR,Western blot和ELISA的cGAS/STING通路组件.

主要成果:

具有PTEN阳性的T98G细胞在STING激活时表现出增强的temozolomide反应.
在T98G细胞中,STING激活增加了STING,IRF3,NF-KB和RELA的mRNA水平.
U118MG细胞 (PTEN阴性) 没有表现出改变的temozolomide反应或通路基因表达.

结论:

刺痛激动剂可以提高temozolomide在PTEN阳性质母细胞瘤中的疗效.
PTEN表达状态可以作为组合治疗的预测生物标志物.
针对STING通路提供了一种潜在的战略,以克服质母细胞瘤的抵抗力.

关键词:

2′3′-c-di-AM(PS) 2 (Rp,Rp) 的时间.在这里,我们可以使用PTEN.刺痛激动剂刺痛激动剂刺痛激动剂在cGAS/STING路径中.质母细胞瘤 (glioblastoma) 是一种泰莫索洛米德 (temozolomide) 是一种有毒的物质.

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