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在正常生理条件下从尿液中调查和注释人类皮层概况

Amr Elguoshy^1,2, Keiko Yamamoto¹, Yoshitoshi Hirao¹

¹Biofluid and Biomarker Center, Kidney Research Center, Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-2181, Japan.

|July 25, 2024

概括

这项研究描述了正常的人类尿体的特征,确定了诸如cathepsin D和矩阵金属蛋白酶-2等关键酶. 这些发现为发现疾病生物标志物奠定了基础.

科学领域:

生物化学生物化学
蛋白质组学是指蛋白质组学.
人体生理学人体生理学

背景情况:

尿管顶反映了生理状态.
改变的皮形状可能表明疾病.
了解正常的尿成分对于生物标志物的发现至关重要.

研究的目的:

为了描述正常的尿.
为了确定产生本源尿的酶.
建立疾病生物标志物研究的参考数据集.

主要方法:

对19个人类尿样进行分析.
内源性的识别和量化.
酶活性概况和与现有数据集的比较.

主要成果:

从436种前体蛋白中确定了1503种内源性.
肝脏和脏基因在体中高度代表.
甲素D (CTSD) 和矩阵金属蛋白酶-2 (MMP2) 是主要的酶.
跨数据集的核心的相对丰度一致.

结论:

建立了正常尿的全面概况.
确定了参与形成的关键酶,CTSD和MMP2.
这些发现为在人类疾病中发现尿路生物标志物提供了基础参考.

关键词:

生物标志物发现发现酸酶的使用方法类药物 (peptidomics) 是一种药物.尿液尿液尿液

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