Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Complement System01:27

Complement System

2.3K
The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a...
2.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Quantitative Proteomic Analysis of Biogenesis-Based Classification for Extracellular Vesicles.

Proteomes·2020
Same author

Proteomic Toolbox To Standardize the Separation of Extracellular Vesicles and Lipoprotein Particles.

Journal of proteome research·2018
Same author

Assessment of Extracellular Vesicles Purity Using Proteomic Standards.

Analytical chemistry·2017
查看所有相关文章

相关实验视频

Updated: Jun 19, 2025

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

4.4K

对与细胞外囊泡相关的补膜攻击复杂蛋白质的定量分析.

Illarion V Turko1

  • 1Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology, University of Maryland, Rockville, ML 20850, USA.

Proteomes
|July 25, 2024
PubMed
概括
此摘要是机器生成的。

细胞外囊泡 (EVs) 可以携带膜攻击复合体 (MAC) 蛋白质,但似乎不包含预先形成的MACs. 这一发现影响了对补充通路中EV介导的细胞间通信的理解.

关键词:
细胞外囊泡中的细胞外囊泡.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量膜攻击复杂的复杂的攻击.多重反应监测多重反应的监测.有针对性的蛋白质量定量.

更多相关视频

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

697
Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

13.7K

相关实验视频

Last Updated: Jun 19, 2025

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity
07:26

Size Exclusion Chromatography to Analyze Bacterial Outer Membrane Vesicle Heterogeneity

Published on: March 31, 2021

4.4K
Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques
08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

697
Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media
08:32

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

Published on: February 12, 2019

13.7K

科学领域:

  • 免疫学 免疫学 免疫学
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • 细胞外囊泡 (EVs) 是细胞间通信的关键媒介.
  • 在EV制剂中检测到补充蛋白,包括形成膜攻击复合体 (MAC) 的蛋白.
  • 电动汽车上MAC蛋白的确切作用和形式尚不清楚.

研究的目的:

  • 在从正常人血中提取的高度净化的EV上调查MAC形成蛋白质的存在和胆量测量.
  • 为了确定EV是否携带预制MAC结构.

主要方法:

  • 量化质谱法用于向蛋白质的测量.
  • 分析的重点是MAC形成蛋白:C5b,C6,C7,C8和C9.
  • 电动汽车从正常人体血中净化.

主要成果:

  • 在纯净的EV中量化了MAC形成蛋白 (C5b-C9).
  • 这些蛋白质在大约相同的静态度下被发现.
  • 观察到的静脉度与预制MAC的预期组成不一致.

结论:

  • 与MAC蛋白相关的EV可以从血中释放出来.
  • 这些EV可以将MAC蛋白传递给受体细胞.
  • 没有证据表明电动汽车携带预制的MAC.