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Updated: Jun 19, 2025

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
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双位移保持β-Kdo糖基转移酶WbbBB的分子机制

Deming Rao1, Lin Zhu1, Weiqiong Liu2,3,4

  • 1School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, People's Republic of China.

The journal of physical chemistry. B
|July 25, 2024
PubMed
概括

这项研究研究了保留糖系转移酶 (GTs) 的催化机制,特别是酶WbbB. 结果揭示了涉及Asp232和Glu158的双位移机制,与常见的GT路径不同.

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This lesson deals with the crossed aldol reaction using weak bases. The self-condensation of an aldehyde having α hydrogen is prevented by adding it slowly to a mixture of formaldehyde and weak bases like hydroxide and alkoxide. Upon slow addition of the aldehyde, the base deprotonates the α carbon of the aldehyde to form the corresponding enolate. The enolate subsequently attacks the formaldehyde to form a single crossed product. Figure 1 depicts the aforementioned reaction.
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