相关实验视频

Updated: Jun 19, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

鉴定和分析表皮细胞-介质细胞转换相关的关键长非编码RNAs在hypospadias

Hongjie Gao¹, Chen Ding², Mengmeng Chang²

¹Department of Pediatrics, Qilu Hospital of Shandong University, Jinan, Shandong, China.

IET systems biology

|July 25, 2024

概括

表皮-介质细胞过渡 (EMT) 功能障碍驱动下. 这项研究确定了GNAS-AS1作为参与EMT的关键长非编码RNA (lncRNA),为hypospadias治疗提供了潜在的治疗标.

科学领域:

发展生物学发展生物学
分子生物学分子生物学
遗传学遗传学是一种遗传学.

背景情况:

表皮-介质细胞过渡 (EMT) 功能障碍是潜在的hypospadias的主要机制.
识别与EMT相关的长非编码RNA (lncRNAs) 对于发现新型分子标记物和低血压症的治疗点至关重要.

研究的目的:

为了识别和表征EMT相关的lncRNAs在hypospadias.
构建一个竞争的内源RNA (ceRNA) 网络,其中包括lncRNA,microRNA (miRNA) 和信使RNA (mRNA).
验证关键的lncRNAs在hypospadias致病的作用,并探索它们的治疗潜力.

主要方法:

下载的基因表达综合 (GEO) 微阵列数据用于低血压症.
选了与EMT相关的差异表达的lncRNA和mRNA.
使用生物信息学构建了lncRNA-mRNA联合表达网络和ceRNA调节网络.
使用mRNA表达数据和细胞功能检测验证了与EMT相关的核心lncRNAs.

主要成果:

确定了6个与EMT相关的lncRNA和123个mRNA-lncRNA联合表达对.
构建了一个包含17个mRNA,4个lncRNA和28个miRNA的ceRNA网络.
经验证的GNAS-AS1和BEX1相关性 (r = 0.874,P < 0.01).
证明GNAS-AS1干扰显著促进细胞增殖,迁移和EMT.

结论:

GNAS-AS1充当ceRNA,并在低血压症患者的EMT中发挥重要作用.
GNAS-AS1代表了对低血压病的潜在治疗标.

关键词:

数据挖掘是数据挖掘的一个方法.疾病疾病的疾病疾病的疾病.

更多相关视频

Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

LINE-1 Methylation Analysis in Mesenchymal Stem Cells Treated with Osteosarcoma-Derived Extracellular Vesicles

Published on: February 1, 2020

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

Metastasis

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...