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相关概念视频

Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

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Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

9.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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相关实验视频

Updated: Jun 19, 2025

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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一种有效的基于深度学习的方法,用于在基因表达中识别拼接位.

Mohsin Ali1, Dilawar Shah1, Shahid Qazi1

  • 1Department of Computer Science, Bacha Khan University, Charsadda, KP, Pakistan.

Science progress
|July 25, 2024
PubMed
概括

这项研究引入了一种使用长短期记忆 (LSTM) 网络的深度学习方法,用于在mRNA序列中准确识别拼接位置. 该方法通过快速准确地绘制点突变来增强基因表达分析.

关键词:
人工智能的人工智能是人工智能.对RNA分析进行RNA分析.生物医学数据 生物医学数据深度学习是一种深度学习.基因组学就是基因组学.拼接站点 拼接站点

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科学领域:

  • 分子生物学分子生物学
  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学

背景情况:

  • 细胞基因表达依赖mRNA拼接,这是一个复杂的过程,涉及spliceosome去除内子和加入外子.
  • 准确识别拼接序列部位对于功能性mRNA生成至关重要,但在当前的方法中仍然具有挑战性.
  • 现有的拼接部位检测技术往往耗时,缺乏复杂基因组分析所需的精度.

研究的目的:

  • 开发一种精确可靠的计算机辅助诊断 (CAD) 技术,以快速识别拼接部位序列.
  • 利用深度学习,特别是长短期记忆 (LSTM) 网络,提高拼接位置检测的准确性.
  • 将拟议的LSTM框架的性能与传统的机器学习和其他深度学习模型进行比较.

主要方法:

  • 使用长短期记忆 (LSTM) 网络的深度学习框架被设计用于拼接位置序列识别.
  • 该网络使用一次性编码来表示RNA序列并提取不同的序列模式.
  • 通过对LSTM与传统机器学习,1D-CNNs和RNNs进行比较的废弃研究来评估性能.

主要成果:

  • 与现有方法相比,拟议的基于LSTM的框架在识别拼接部位序列方面表现出卓越的性能.
  • LSTM网络实现了改进的准确性,接受站点增加了3%,捐赠站点数据集增加了2%.
  • 深度学习方法有效地绘制了点突变,并确定了RNA序列中的关键拼接模式.

结论:

  • 开发的深度学习CAD技术为真核生物的结合部位识别提供了准确和快速的解决方案.
  • LSTM网络为提取RNA序列中的复杂模式提供了强大的工具,大大提高了拼接位点检测的准确性.
  • 计算生物学的进步可以加速基因表达的研究和理解与拼接错误相关的遗传疾病.