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在感染Mycobacterium bovis的小鼠中,LCN2调节肠道微生物群和代谢特征

Quntao Huang¹, Junhong Xing², Guoli Li³

¹MOE Joint International Research Laboratory of Animal Health and Food Safety, Key Laboratory of Animal Bacteriology, Ministry of Agriculture, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.

|July 25, 2024

概括

缺乏利波卡林2 (Lcn2) 会改变感染Mycobacterium bovis的小鼠的肠道微生物群和新陈代谢. 这突显了Lcn2在感染期间维持肠道健康方面的作用.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
胃肠病学胃肠病学

背景情况:

牛肉菌菌 (Mycobacterium bovis) 感染会导致牛的重大病理.
利波卡林2 (Lcn2) 是炎症反应和组织损伤的关键调解剂.
在M. bovis感染期间,Lcn2在调节肠道环境中的特定作用尚不清楚.

研究的目的:

为了研究Lcn2在塑造肠道微生物群和M. bovis感染后小鼠的代谢资料中的作用.
评估Lcn2缺乏在感染后5周内对肠道环境的影响.
为了确定与缺乏Lcn2.2相关的特定微生物和代谢变化.

主要方法:

使用Lcn2淘汰 (Lcn2-/-) 小鼠和野生类型的对照.
感染了Mycobacterium bovis的小鼠并被监测了5周.
分析了肠道微生物群组成 (OTU,α和β多样性) 和代谢概况.

主要成果:

感染M. bovis的Lcn2-/-小鼠显示肠道微生物群组成和多样性的显著变化.
在Lcn2-/-小鼠中观察到Akkermansia,Oscillospira和Bacteroides的丰度增加,而Ruminococcus的数量减少.
缺少Lcn2导致肠道代谢概况发生变化,特定脂肪酸和胆汁酸水平升高.

结论:

在M. bovis感染期间,Lcn2在调节肠道微生物群和代谢组方面发挥着至关重要的作用.
缺少Lcn2显著改变肠道环境,影响微生物和代谢平衡.
这些发现表明Lcn2是治疗策略的潜在目标,旨在减轻M. bovis感染中的肠道失调症.

关键词:

Lcn2 在线观看在M. bovis中发现了M.我们的肠道微生物群.非向的代谢组.

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