血蛋白质组概况与极度早产婴儿肺损伤的早期发展有关
Prue M Pereira-Fantini1,2,3, Sean G Byars4, C Omar F Kamlin3
1Neonatal Research Group and.
概括
在极度早产的婴儿中,可以及早检测支气管肺功能障碍症 (BPD). 血中的蛋白质生物标志物,在出生后72小时内确定,显示出预测BPD发展的潜力.
科学领域:
- 新生儿医学 新生儿医学
- 生物标志物发现发现
- 呼吸系统健康 呼吸系统健康
背景情况:
- 极度早产婴儿的肺损伤媒介在很大程度上是未知的,阻碍了早期诊断和治疗.
- 支气管肺功能障碍症 (BPD) 是极度早产婴儿的重大并发症,需要改进预测策略.
研究的目的:
- 为了确定血中的蛋白质变化从极度早产婴儿预测BPD.
- 评估血蛋白生物标志物在早期BPD预测中的潜力.
主要方法:
- 利用从生命的第一个72小时开始的等离子体样本上所有理论质谱 (SWATH-MS) 的顺序窗口获取.
- 采用生物信息分析来识别与BPD相关的生物途径.
- 使用后勤回归与引导重新抽样来评估蛋白质生物标记物的预测潜力与出生特征相结合.
主要成果:
- 在婴儿中观察到凝血,补充激活,发育和细胞外矩阵组织的变化,这些婴儿后来发展为BPD.
- 确定了49种血蛋白质,这些蛋白质具有对BPD结果的预测潜力 (乐观度调整后的C指数为0.65-0.84).
- 在患有BPD的婴儿中,早在4小时就检测到血蛋白质的改变.
结论:
- 血蛋白质的改变可以在极度早产的婴儿中早期检测到,这些婴儿患有BPD.
- 蛋白质生物标志物与出生特征相结合,显示出预测生命前72小时内BPD的前途.
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