在生理氧含量下培养人类肠道有机体的新系统,以研究微生物与宿主相互作用
Tatiana Y Fofanova1, Umesh C Karandikar1, Jennifer M Auchtung1,2
1Alkek Centre for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States of America.
PloS one
|July 25, 2024
概括
一个新的肠道有机体生态培养 (IOPC) 系统使无氧肠道微生物与人类肠道有机体共同培养成为可能. 这一突破推动了对宿主微生物相互作用和微生物群的理解.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 细胞生物学 细胞生物学
背景情况:
- 研究人类肠道中宿主-微生物相互作用是具有挑战性的,因为无氧肠道细菌和依赖氧气的肠道上皮细胞之间的氧气需求不同.
- 现有的共同培养系统努力复制肠道环境中存在的独特氧气梯度.
- 专门的共同培养系统对于准确研究肠道微生物群和肠道组织之间的复杂相互作用至关重要.
研究的目的:
- 开发和验证一种新的肠道有机体生态菌培养 (IOPC) 系统.
- 在生理学上相关的条件下,使无氧肠道细菌与人类肠道有机体 (HIOs) 能够共同培养.
- 研究宿主微生物相互作用及其对肠道屏障功能和免疫反应的影响.
主要方法:
- 开发肠道有机体生态培养 (IOPC) 系统,这是一个具有成本效益的方法,用于创建对比的氧气环境.
- 在24-48小时内与无氧共生细菌 (包括Bacteroides thetaiotaomicron和Blautia sp.) 共同培养人类肠道有机体 (HIOs).
- 评估HIO屏障完整性,基因表达 (包括免疫调节基因) 和转录组分析来自供体的HIO反应.
主要成果:
- IOPC系统成功地支持了HIO与无氧细菌的共同培养,模仿了体内氧气条件.
- 在IOPC培养的HIO表现出增强的屏障完整性和免疫调节基因的增加表达.
- 转录组分析显示,HIO对无氧细菌共同培养的反应具有捐助者特异性的变异.
结论:
- 肠道有机体生态培养 (IOPC) 系统为研究宿主微生物相互作用提供了一个强大的和易于访问的平台.
- 该系统利用患者获得的肠道组织,促进对肠道微生物群在健康和疾病中的作用的研究.
- 通过IOPC,可以对肠道细菌如何影响肠道生理学和免疫力进行机械研究.
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