相关实验视频
Updated: Jun 19, 2025

04:07
Isolation of Epithelial Cells from Human Dental Follicle
Published on: November 5, 2021
2.8K
Pitx2和Tfap2a/Tfap2b的转录程序控制了牙启动期间下上皮质的谱系规范
Fan Shao1,2, An-Vi Phan1, Wenjie Yu3
1Iowa Institute for Oral Health Research, University of Iowa College of Dentistry and Dental Clinics, Iowa City, Iowa, United States of America.
PLoS genetics
|July 25, 2024
概括
研究人员确定了控制部发育的关键转录因子 (TFs). 破坏这些TF改变了牙的形成,揭示了脊椎动物下巴和牙开始部位的模式所必不可少的交叉抑制机制.
科学领域:
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 确定脊椎动物下的背腹轴,特别是牙开始部位,是一个至关重要的未解决的问题.
- 牙的发育源于牙膜,牙中的一个专门的上皮结构.
研究的目的:
- 确定规范牙膜规范的转录性调节网络 (TRN).
- 了解下上皮质的背腹轴模式的分子机制,并调节牙启动.
主要方法:
- 利用激光微切割与低输入RNA测序 (LMD-RNA-seq) 结合,在下上皮层域中分析基因表达.
- 对Pitx2,Tfap2a和Tfap2b等关键转录因子 (TF) 的功能丧失小鼠模型进行了检查.
- 分析了介质细胞信号通路 (SHH和WNT) 与上皮TFs的相互作用.
主要成果:
- 确定了TFs Sox2和Tfap2 (Tfap2a/Tfap2b) 沿背腹轴的互补表达域.
- 域特定TF的破坏导致了替代转录监管网络的升级监管和扩展.
- 在Tfap2a/Tfap2b淘汰中观察到异位牙形成,这表明交叉抑制的重要性.
- 在牙启动部位发现了介质细胞SHH/WNT信号与上皮TFs之间的接口.
结论:
- 一种涉及域特异TFs (Pitx2,Tfap2a/Tfap2b) 交叉抑制的新型分子机制在小鼠下的背腹轴上形成了模式.
- 这种机制对于调节牙开始的精确位置至关重要.
- 这些发现为脊椎动物的下模式和牙发育提供了基本的见解.
相关概念视频
Determination
18.3K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
18.3K
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
General Transcription Factors
5.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.2K
TGF - β Signaling Pathway
7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K
Lineage Commitment
3.0K
Commitment is the process whereby stem cells:
3.0K
Transcription Factors
75.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.8K

