含有激进SAM酶的阿西诺素生物合成,用于非正规SAM裂变和C-As键形成
Yadi Yao1, Jiale He1, Fan Chen1
1Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
Journal of the American Chemical Society
|July 25, 2024
概括
阿西诺素抗生素生物合成涉及一种独特的激进SAM酶,ArsL,它形成碳键. 这项研究阐明了ArsLL.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 微生物学 微生物学
背景情况:
- 阿西诺素是一种由土壤细菌产生的强效抗生素.
- 它的生物合成包括形成由蛋白质ArsL催化的一种碳键.
- ArsL 含有 CxxxCxxC 基因,这是 SAM 激进酶的特征.
研究的目的:
- 描述了激素SAM酶ArsL在氨酸生物合成中的功能和机制.
- 与其他激进SAM酶相比,研究ArsL的独特特征.
- 在体外 (in vitro) 复制阿西诺素生物合成.
主要方法:
- 酶试验以确定ArsL在S-adenosyl-l-methionine (SAM) 上的裂变活性.
- 针对位点的突变发生和截断研究,以确定ArsL.中的关键功能动机.
- 在实验室中使用ArsL和ArsM进行arsinothricin生物合成的复制.
主要成果:
- 阿尔斯L分裂SAM产生3 - 氨基-3 - 碳酸 (ACP) 基,这是一个非正规的反应激进SAM酶的CxxxCxxC动机.
- 在ArsL中保存的RCCLKC基因对酸盐结合和酶活性至关重要.
- 在实验室中,ArsL和ArsM成功地重建了arsinothricin生物合成.
结论:
- ArsL代表了一个独特的激进SAM酶,表现出正规的动机,但催化了一种非正规的反应.
- 这些发现表明,在这种酶超级家族中,可能存在未被发现的非正规基的SAM化学反应.
- 这项研究提供了详细的机械洞察力,对氨酸生物合成.
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