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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
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一种新的SERS检测大肠杆菌的新方法,基于一个aptamer功能化的Au-Ag@Si三角形金字塔基板.

Xiaochen Qu1, Guohui You1, Shufang Wang2

  • 1College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
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概括

一种新的基于aptamer的表面增强拉曼光谱法 (SERS) 方法可以快速和灵敏地检测大肠杆菌 (E. coli). 这种技术为在药品和食品样本中现场检测大肠杆菌提供了一个有希望的替代方案.

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亚普特美尔 (Aptamer) 是一种药物.在Au-Ag@Si三角形金字塔基板.埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.表面增强的拉曼光谱学

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科学领域:

  • 分析化学 分析化学
  • 生物感应是一种生物感应.
  • 纳米技术纳米技术

背景情况:

  • 大肠杆菌 (E. coli) 污染对食品和药品构成重大风险,需要严格的监管控制.
  • 目前用于检测大肠杆菌的方法往往缺乏有效监测所需的速度和灵敏度.
  • 开发快速和灵敏的检测技术对于确保公共卫生和产品安全至关重要.

研究的目的:

  • 开发一种基于aptamer的表面增强拉曼光谱 (SERS) 三明治方法,用于快速和灵敏地检测大肠杆菌.
  • 使用aptamer功能化的金-银@三角金字塔 (Au-Ag@Si TP) 基板进行增强的大肠杆菌检测.
  • 为了验证该方法在检测大肠杆菌在复杂的矩阵如制药制剂中的有效性.

主要方法:

  • 制造一个胺基功能化的Au-Ag@Si TP基质,同时作为捕获探头和SERS标签.
  • 整合一个拉曼记者 (6-carboxy-X-rhodamine) 用于信号放大.
  • 开发一种选择性大肠杆菌捕获和检测的三明治测试格式.

主要成果:

  • 运用aptamer功能化的Au-Ag@Si TP基质被快速制造 (20.5小时).
  • 开发的方法在约3小时内实现了选择性和快速的大肠杆菌检测,检测极限为2.8CFU/mL.
  • 对新黄片的成功应用显示了高恢复率 (90.19%-104.17%),表明了强度.

结论:

  • 基于aptamer的SERS三明治方法为大肠杆菌检测提供了一种简单,快速和敏感的方法.
  • 这种技术显示出作为在制药和食品工业现场监测大肠杆菌的替代方案的巨大潜力.
  • 使用功能化的Au-Ag@Si TP基板可以提高细菌污染物的检测能力.