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GAS6-AS1通过增加MMP7表达以ceRNA和RBP依赖的方式驱动膀癌的进展

Xinyi Zhou¹, Linmei Xiao², Fanyu Meng³

¹Department of General Surgery, Affiliated Hospital of Jiangnan University, 1000 Hefeng Road, Wuxi, 214000, China.

Translational oncology

|July 25, 2024

概括

长非编码RNA GAS6-AS1通过调节细胞增殖和侵入,促进尿道膀癌 (UBC) 的进展. GAS6-AS1通过两个关键途径起作用,为UBC诊断和治疗提供了潜力.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

长非编码RNA (lncRNAs) 在各种疾病中发挥着关键作用.
在尿膀癌 (UBC) 中 lncRNAs 的特定功能需要进一步研究.

研究的目的:

阐明lncRNAs在UBC进展中的机制.
确定和描述GAS6-AS1在UBC中的作用.

主要方法:

在线数据库的生物信息分析,以确定UBC中差异表达的lncRNAs.
在体外和体外功能实验中评估GAS6-AS1.1.的作用.
分子生物学技术包括RNA拉下,双露西法酶记者基因测试,RNA免疫沉降 (RIP),FISH和三重捕获测试以确定分子相互作用.

主要成果:

GAS6-AS1在UBC组织中高度表达,促进了UBC细胞的增殖,迁移,入侵和细胞周期进展.
GAS6-AS1与miR-367-3p和PRC1.1.进行相互作用.
GAS6-AS1通过隔离miR-367-3p来增强MMP7表达,并通过抑制PRC1结合通过APC转录来激活Wnt通路瘤基因.

结论:

GAS6-AS1通过GAS6-AS1 / miR-367-3p / MMP7和GAS6-AS1 / PRC1 / APC / Wnt / MMP7轴促进了UBC的进展.
GAS6-AS1是UBC诊断,治疗和预后的潜在生物标志物.

关键词:

细胞粘附和运动性/转移相关基因通过表达分析 (mRNA,蛋白) 进行诊断.微RNA / 非编码RNA 的编码.尿道器官尿道器官没有信号传递相关基因.

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