通用树脂复合水泥的细胞毒性和微生物行为
Uros Josic1, Gabriella Teti1, Andrei Ionescu2
1Department for Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy.
概括
选择通用树脂复合水泥 (UCs) 及其聚合方法显著影响牙纸细胞活力和Streptococcus mutans生物膜形成. 光固化UCs可以减少对牙纸细胞的潜在伤害.
科学领域:
- 牙科材料科学 牙科材料科学
- 生物材料是一种生物材料.
- 微生物学 微生物学
背景情况:
- 通用树脂复合水泥 (UCs) 越来越多地用于牙科修复.
- 了解它们的细胞毒性和对口腔细菌的影响对于临床成功至关重要.
- 以前的研究集中在特定的水泥类型,但需要对UC进行比较分析.
研究的目的:
- 在人类牙纸细胞 (HDPCs) 上评估三种UC的细胞毒性.
- 评估UCs对Streptococcus mutans (S.mutans) 生物膜形成的影响.
- 为了比较光固化 (LC) 与自我固化 (SC) 协议对UCs生物行为的影响.
主要方法:
- 在LC和SC条件下测试了三个UC和一个黄金标准水泥.
- 细胞毒性使用7天的MTT试验进行了评估.
- 测量了反应性氧物种 (ROS) 和互乐金-6 (IL-6) 释放.
- 在24小时后评估了S.mutans生物膜的形成,一些样本经历了加速老化.
主要成果:
- 细胞毒性在UCs和聚合方案之间有显著的差异,自固化 (SC) 水泥通常具有更高的毒性.
- 在第3天和第7天观察到IL-6的表达增加,这表明炎症反应.
- 对于特定的SC和LCUC,ROS释放量最高.
- 与SC相比,光固化 (LC) 显著减少了S.mutans生物膜的形成,约为33%,与SC相比.
结论:
- 水泥品牌和聚合协议都对HDPC的生存能力和S.mutans生物膜产生了重大影响.
- 光固化是UCs的一个有益的协议,可以减轻潜在的脉毒性和细菌粘附.
- 临床选择的UC应该考虑聚合方法,以获得最佳的患者结果.
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