加列-3上调的FAK通过口腔平活化纤维细胞促进血管生成
Xiaoheng Xu1, Qian Mi1, Siting Chen1
1Department of Oral Medicine, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou, Guangdong Province, China.
概括
口腔平坦状体 (OLP) 纤维细胞通过增加加勒-3.3促进血管生长 (血管生成). 在OLP纤维细胞中抑制加列-3可降低这种效应,这表明OLP的治疗点.
科学领域:
- 口腔病理学 口腔病理学
- 细胞生物学 细胞生物学
- 血管新生研究研究
背景情况:
- 口腔平活化纤维细胞对血管新生有所贡献的机制尚未完全理解.
- 这项研究研究了加勒-3在口腔平坦 (OLP) 中的作用及其通过OLP激活纤维细胞促进血管生成的潜力.
研究的目的:
- 为了确定OLP组织和OLP激活纤维细胞中的加列-3的表达.
- 调查加勒-3是否通过OLP激活纤维细胞促进血管生成.
- 探索抑制OLP相关血管生成中的galactin-3的治疗潜力.
主要方法:
- 免疫组织化学用于检测OLP和正常口腔粘膜组织中的加列-3和CD34表达.
- 在OLP激活的纤维细胞中,用RT-PCR,西斑和ELISA量化了盖莱克-3的表达.
- 使用OLP激活纤维细胞和人静脉血管内皮细胞的共同培养进行了功能性测试 (Transwell,管形成),有或没有加列-3调制 (过度表达或GB1107抑制).
主要成果:
- 加勒-3在OLP组织中表达高,与血管生成有积极的相关性.
- 与正常纤维细胞相比,OLP激活的纤维细胞表现出显著更高的加列-3表达.
- 在OLP激活的纤维细胞中,加勒-3的过度表达增强了内皮细胞迁移和管形成. 抑制加勒-3可降低这些效应,并降低关键血管生成因子 (VEGF,FGF2) 和信号通路 (VEGFR2/FAK).
结论:
- 在OLP中升级调节的加列-3与血管生成有关.
- 由OLP激活的纤维细胞通过Galectin-3促进内皮细胞迁移和分化,通过VEGFR2/FAP信号传递起作用.
- 向加勒-3可能提供一种治疗策略,用于管理OLP中的血管生成.
相关概念视频
Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
Intracellular Signaling Affects Focal Adhesions
2.6K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Some...
2.6K
Mechanism of Angiogenesis
5.4K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.4K
Laminins are the Adhesive Proteins of Basal Lamina
2.1K
Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
2.1K


