在压力下增强蛋白质稳定性:以奥斯莫利特为基础的深溶解剂作为热和冷冲击下的酶生物相容和强大的稳定介质
Anja Damjanović1, Marijan Logarušić1, Lidija-Marija Tumir2
1Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia. mcvjetko@pbf.hr.
Physical chemistry chemical physics : PCCP
|July 26, 2024
概括
含有天然氧化物的深度浸泡溶剂 (DES) 在恶劣条件下有效地稳定了酶等蛋白质. 基于氨酸和贝他因的DES与糖醇显示出优异的蛋白质稳定性,并被发现是无毒的.
科学领域:
- 生物化学 生物化学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 液体蛋白质配方在生物技术中至关重要,但面临着稳定性挑战.
- 环境因素可以降低溶液中的蛋白质完整性.
- 深度浸泡溶剂 (DES) 具有稳定蛋白质配方的潜力.
研究的目的:
- 为了研究以奥斯莫利特为基础的DES用于增强蛋白质稳定.
- 在各种存储条件下探索酶的结构变化.
- 评估生物灵感DESs对热和冷-解压力的有效性.
主要方法:
- 选DESs与宇宙热性结体 (例如,TMAO,贝他因,撒可) 和糖醇或尿素.
- 将溶酶溶液置于热冲击 (80°C) 和冷解周期 (-20°C, -80°C) 中.
- 使用生物试验,聚合试验,FT-IR和CD光谱学监测酶活性,体稳定性和构造变化.
主要成果:
- 基于奥斯莫利特的DES在压力下显示出显著的酶稳定.
- 以糖醇为基础的沙科辛和贝他因DES是最有效的,即使在高蛋白度 (200毫克/毫升) 中也是如此.
- 在kosmotrope-urea混合物中观察到协同效应,在kosmotrope-glycerol混合物中观察到累积效应.
结论:
- 基于奥斯莫利特的DES在具有挑战性的储存条件下为蛋白质提供了强大的稳定性.
- 在DES中的水含量影响了酶活性和稳定性之间的平衡.
- 经过测试的基于奥斯莫利特的DES对人类细胞系没有毒性,这表明生物医学应用的安全性.
更多相关视频
07:22How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
Published on: February 11, 2019
28.2K
07:59Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
Published on: June 29, 2021
3.5K
相关概念视频
Responses to Heat and Cold Stress
15.8K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
15.8K
Factors Influencing Microbial Growth: Osmolarity
1.5K
Osmolarity is the measure of solute concentration in a solution. It plays a critical role in determining water availability for organisms. Water moves across semipermeable membranes through osmosis, flowing from regions of lower solute concentration (more dilute) to regions of higher solute concentration (more concentrated).In high-solute environments, microbial cells lose water, leading to dehydration and inhibited growth. The extent to which water is available to microbes in such environments...
1.5K
Other Stress Responses in Bacteria
541
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
541
Diversity of Archaea III
462
Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...
462
Diversity of Archaea IV
611
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
611
