脉冲低级激光对成人的效果与新生儿人类视网膜色素上皮细胞的效果:一个体外研究
Seyed Mohamadmehdi Moshtaghion1,2, Mohammad Abolhosseini1, Faraj Tabeie3
1Ocular Tissue Engineering Research Center, Research Institute for Ophthalmology and Vision Science, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Journal of ophthalmic & vision research
|July 26, 2024
概括
低级激光器 (LLL) 显示出诱导视网膜细胞多功能的潜力. 虽然新生儿细胞面临肌纤维细胞转化风险,但780nm激光为成年视网膜退化治疗提供了一个有希望的低风险选择.
科学领域:
- 眼科医生 眼科 眼科
- 细胞生物学 细胞生物学
- 生物光子学 生物光子学
背景情况:
- 人类视网膜色素上皮质 (hRPE) 细胞对于视网膜健康至关重要.
- 低级激光 (LLLs) 或低功率激光疗法正在被用于治疗应用.
- 了解LLL对hRPE细胞的影响对于潜在的视网膜疾病治疗至关重要.
研究的目的:
- 研究LLLs对培养人类视网膜色素上皮细胞 (hRPE) 细胞的结构,遗传学和表型的短期影响.
- 为了比较LLL辐射对成人和新生儿hRPE细胞的影响.
主要方法:
- 培养成人的hRPE细胞和新生儿hRPE细胞每天连续五天用630nm和780nm的LLLs进行辐射.
- 细胞翻倍时间,基因表达特征和蛋白质表达经过辐射后被分析.
主要成果:
- 在用630nmLLL (P = 0.032) 照射成年hRPE细胞中观察到翻倍时间的显著增加.
- 基因表达分析显示,在新生儿和成人hRPE细胞中,关键基因的差异调节,包括RPE65,nestin,PAX6和ACTA2.
- 蛋白质表达的显著变化很小,除了新生儿hRPE细胞中α-SMA的增加外,新生儿hRPE细胞被照射了780nmLLL.
结论:
- 短期LLL辐射可能会在新生儿和成人hRPE细胞中诱导转录多能性.
- 用LLL照射的新生儿hRPE细胞显示肌纤维细胞转化风险增加.
- 780nm激光似乎是一个有前途的,低风险的选择,用于成年视网膜退化中的光生物调节.
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